FastQCFastQC Report
Thu 26 May 2016
SRR1512337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78700.4068806779759159No Hit
TATCAACGCAGAGTACTTTTTTTTT47900.24764402128394378No Hit
GGTATCAACGCAGAGTACTTTTTTT46440.2400957901550386No Hit
GTCCTACAGTGGACATTTCTAAATT28510.14739730786649763No Hit
CTGTAGGACGTGGAATATGGCAAGA27720.14331299102277498No Hit
GTCCTAAAGTGTGTATTTCTCATTT27200.14062457993576766No Hit
CTTTAGGACGTGAAATATGGCGAGG25150.13002603622737338No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23100.11942749251897915No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19900.10288342429124178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGA400.00523812814.2669971
GGTATCA11700.014.1450571
TAGGACC4900.011.8305264
GCACCGT907.4222335E-611.61683756
GTATCAA28150.011.2514161
GTACGGA600.005824623611.0965531
AATCCCG951.3703237E-510.99290619
GGACCGT700.001485564410.8624966
AAGACGT1002.3882303E-510.4543415
GGCGAGG6650.010.421844519
TTAGGAC9500.010.2034453
ACTATCG2350.010.10535111
TAGACAG2850.010.0041545
AGGACGT16600.09.90471655
GGTCTAC2550.09.6978281
GGACGTG16500.09.6774976
TGGCGAG15700.09.675034518
TCCTACA15250.09.5343682
GTATTAA2303.6379788E-129.5113321
GTCCTAC14700.09.5113321
Produced by FastQC (version 0.11.5)