FastQCFastQC Report
Thu 26 May 2016
SRR1512336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632460
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73310.4490768533379072No Hit
GTCCTACAGTGGACATTTCTAAATT57580.35271920904646975No Hit
GTCCTAAAGTGTGTATTTCTCATTT54610.33452580767675777No Hit
CTGTAGGACGTGGAATATGGCAAGA53070.3250921921517219No Hit
CTTTAGGACGTGAAATATGGCGAGG47550.2912781936463987No Hit
TATCAACGCAGAGTACTTTTTTTTT42380.2596081986694927No Hit
GGTATCAACGCAGAGTACTTTTTTT42260.2588731117454639No Hit
GTCCTACAGTGTGCATTTCTCATTT33580.20570182424071645No Hit
CTGAAGGACCTGGAATATGGCGAGA22970.1407078887078397No Hit
CTGTAGGACCTGGAATATGGCGAGA21980.13464342158460238No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20850.12772135304999815No Hit
ATTTAGAAATGTCCACTGTAGGACG20710.12686375163863126No Hit
GTCCTTCAGTGTGCATTTCTCATTT20210.12380088945517809No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19820.12141185695208458No Hit
TTTCTAAATTTTCCACCTTTTTCAG19720.12079928451539394No Hit
GAATATGGCAAGAAAACTGAAAATC19510.11951288239834361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1301.8189894E-1213.89050818
GGTATCA10250.013.2535721
TAGGACC9050.012.7015754
GGTTAGT905.405891E-712.6665851
GTCCTAG2050.012.0487021
ACGGCGA657.9458323E-411.70591817
GGACGTC658.0262776E-411.6918726
GGCGAGG13850.011.46220619
TACGGCG752.051635E-411.41327116
TACTAGC600.005873446411.0839432
GTATCAA22800.010.9165951
TAGACTG1408.352799E-910.8567395
CCAACGA1158.743227E-710.74600419
AGGACCT18300.010.74550155
TAGAAAT8850.010.7343944
AAATGTC8350.010.6945437
ACCTTTT6150.010.67061515
GGACCTG18200.010.5957596
CCACCTT6200.010.57413913
TGTCCAC8250.010.46873510
Produced by FastQC (version 0.11.5)