FastQCFastQC Report
Thu 26 May 2016
SRR1512336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632460
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90350.5534591965499921No Hit
CTGTAGGACGTGGAATATGGCAAGA57510.3522904083407863No Hit
GTCCTACAGTGGACATTTCTAAATT57360.3513715496857503No Hit
TATCAACGCAGAGTACTTTTTTTTT54020.33091163030028303No Hit
GTCCTAAAGTGTGTATTTCTCATTT53210.32594979356308884No Hit
GGTATCAACGCAGAGTACTTTTTTT52870.32386704727834065No Hit
CTTTAGGACGTGAAATATGGCGAGG50770.31100302610783725No Hit
GTCCTACAGTGTGCATTTCTCATTT33170.20319027725028485No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27590.16900873528294721No Hit
CTGAAGGACCTGGAATATGGCGAGA26520.16245421021035739No Hit
CTGTAGGACCTGGAATATGGCGAGA23580.1444445805716526No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22230.13617485267632898No Hit
ATTTAGAAATGTCCACTGTAGGACG22150.13568479472697645No Hit
TTTCTAAATTTTCCACCTTTTTCAG21090.12919152689805571No Hit
GAATATGGCAAGAAAACTGAAAATC20880.12790512478100535No Hit
GTCCTTCAGTGTGCATTTCTCATTT19490.11939036791100548No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18740.11479607463582568No Hit
GGAATATGGCGAGAAAACTGAAAAT17080.10462737218676109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.8711711
GTCGAGG707.2930197E-613.56602419
TAGGACC9200.013.1173454
CTGCGAT658.001137E-411.696259
TGCGATA658.0382527E-411.68979910
GTGGTAT3600.011.6253611
GTCCTAT2650.011.4857781
CACCTTT7050.011.44828814
TGCGCCA700.001494524410.85448212
CCACCTT7200.010.8161313
GTATCAA28200.010.7596441
TAGAAAT8750.010.642614
AATCCCG1252.2519816E-710.63576319
GGCGAGG12500.010.63576319
AAGTCGT2150.010.6088777
GTACTGG5300.010.5884511
ACCTTTT8000.010.5635715
AGGACCT18700.010.5189445
AGTCGTC2350.010.5148288
GTATTAT1553.587047E-910.4321421
Produced by FastQC (version 0.11.5)