FastQCFastQC Report
Thu 26 May 2016
SRR1512335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825980
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61490.3367506763491385No Hit
GTCCTAAAGTGTGTATTTCTCATTT46910.2569031424221514No Hit
GTCCTACAGTGGACATTTCTAAATT46810.25635549129782365No Hit
CTGTAGGACGTGGAATATGGCAAGA42390.2321493116025367No Hit
CTTTAGGACGTGAAATATGGCGAGG41460.22705615614628857No Hit
GGTATCAACGCAGAGTACTTTTTTT35130.19238983997634146No Hit
TATCAACGCAGAGTACTTTTTTTTT34050.1864752078336017No Hit
GTCCTACAGTGTGCATTTCTCATTT30080.16473345819778967No Hit
CTGTAGGACCTGGAATATGGCGAGA20980.11489720588396368No Hit
CTGAAGGACCTGGAATATGGCGAGA20000.10953022486555165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCG551.9792048E-413.8026719
GTCGTAC500.001501224213.2982281
TTCGATT500.001502158113.29713112
GTTCGAT500.00150465113.29420511
GGTATCA10400.013.2434281
GCACTCG604.086773E-412.66881413
GTGTAGG3400.012.5718551
TAGGACC8550.012.2222764
ATAGGAC1900.011.5003673
GGCGAGG11700.011.45475619
GTATCAA25000.011.3984811
CGATTCC600.00584250411.09192714
AGCTCGT600.005901436311.07698110
CACCTTT6200.010.88746614
AATCCCG1408.283678E-910.86286819
GTCCTAC25650.010.6652461
CAAGTCG2350.010.5101066
TGGCGAG25950.010.50823418
GGACCTG15750.010.3740776
TCCAACG1106.0356433E-610.36510818
Produced by FastQC (version 0.11.5)