##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1825980 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.181127942255664 31.0 30.0 34.0 16.0 34.0 2 30.0950903076704 33.0 30.0 34.0 25.0 34.0 3 30.80016593829067 33.0 31.0 34.0 27.0 34.0 4 34.4671245030066 37.0 35.0 37.0 32.0 37.0 5 34.64825901707576 37.0 35.0 37.0 32.0 37.0 6 34.80014457989682 37.0 35.0 37.0 32.0 37.0 7 34.7408109617849 37.0 35.0 37.0 32.0 37.0 8 34.7379429128468 37.0 35.0 37.0 32.0 37.0 9 36.34501527946637 39.0 37.0 39.0 32.0 39.0 10 36.27649152783711 39.0 37.0 39.0 32.0 39.0 11 36.30360683030482 39.0 37.0 39.0 32.0 39.0 12 36.249847205336316 39.0 37.0 39.0 32.0 39.0 13 36.22712187428121 39.0 37.0 39.0 32.0 39.0 14 37.493084261601986 40.0 38.0 41.0 32.0 41.0 15 37.43160549403608 40.0 38.0 41.0 32.0 41.0 16 37.36656589885979 40.0 38.0 41.0 32.0 41.0 17 37.327871608670414 40.0 38.0 41.0 32.0 41.0 18 37.109876340376125 40.0 38.0 41.0 31.0 41.0 19 37.215165554934885 40.0 38.0 41.0 32.0 41.0 20 37.152329160231766 40.0 38.0 41.0 32.0 41.0 21 37.10807730643271 40.0 38.0 41.0 31.0 41.0 22 37.03415097646195 40.0 38.0 41.0 31.0 41.0 23 36.91436762724674 40.0 38.0 41.0 31.0 41.0 24 36.80002847785847 40.0 38.0 41.0 31.0 41.0 25 36.694986801607904 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1461473062232486 1101 2 0.30834247190129815 1101 3 -0.2395216222028438 1101 4 -0.41307179277857387 1101 5 -0.5654377136583264 1101 6 -0.589443155346622 1101 7 -0.5898566865084902 1101 8 -0.6761639122330934 1101 9 -0.7760426438066617 1101 10 -1.006595938989335 1101 11 -0.9361419692467408 1101 12 -1.1408051480324417 1101 13 -1.249820101418301 1101 14 -1.3457120792175417 1101 15 -1.413664890597225 1101 16 -1.5769839067082572 1101 17 -1.6130068879588038 1101 18 -1.443023048456638 1101 19 -1.3125935909088398 1101 20 -1.4696456378882559 1101 21 -1.5295658525122917 1101 22 -1.574279216891746 1101 23 -1.6952789493725788 1101 24 -1.8608858318149046 1101 25 -1.7996180275614293 1102 1 1.0413261200053725 1102 2 0.13360591402219057 1102 3 -0.21954976059256026 1102 4 -0.44436722455363764 1102 5 -0.6822333479445248 1102 6 -0.5669722352354896 1102 7 -0.5942380327456931 1102 8 -0.6234470654380999 1102 9 -0.7805348382457709 1102 10 -0.9460706890479571 1102 11 -0.8564541916821327 1102 12 -1.0170807905612023 1102 13 -0.8503558677275578 1102 14 -1.3131895010020571 1102 15 -1.0342393116640665 1102 16 -1.4476247656397092 1102 17 -1.5179204476782715 1102 18 -1.3338496491828309 1102 19 -1.4389775946606278 1102 20 -1.598386587061512 1102 21 -1.6434893814642777 1102 22 -1.6523035608622436 1102 23 -1.9215570788802268 1102 24 -2.0116166483256066 1102 25 -1.8979027226933454 1103 1 1.5132803110385105 1103 2 0.5441139549578651 1103 3 0.0637774811228411 1103 4 1.7468577775048288E-4 1103 5 -0.08595532650203097 1103 6 -0.07585381418285664 1103 7 -0.3012494849874088 1103 8 -0.2392791804478449 1103 9 -0.27569809360639397 1103 10 -0.36802488008109435 1103 11 -0.3608036068868117 1103 12 -0.4965033051810863 1103 13 -0.48982955193807953 1103 14 -0.7290642573373418 1103 15 -0.7249617775510089 1103 16 -0.7121045414681646 1103 17 -0.8241521728244763 1103 18 -0.7482685088319556 1103 19 -0.8283050800407423 1103 20 -0.7421975032408454 1103 21 -0.9299416231796798 1103 22 -1.1589921573534738 1103 23 -1.1144116110439697 1103 24 -1.1098683539786265 1103 25 -1.2601529663580635 1104 1 1.1709959305577513 1104 2 0.4732351635620127 1104 3 -0.09231035288108203 1104 4 -0.08595447959933011 1104 5 -0.31843527337090194 1104 6 -0.27407611378050234 1104 7 -0.3115342910330128 1104 8 -0.5130917900630294 1104 9 -0.5677084361216131 1104 10 -0.6762666524941778 1104 11 -0.6397052627209234 1104 12 -0.5216483841040187 1104 13 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1108 21 -1.3214547941046177 1108 22 -1.495656595856147 1108 23 -1.5264418398021462 1108 24 -1.4213799156740095 1108 25 -1.4326308085050243 1109 1 0.9699108905868599 1109 2 0.15857150974956724 1109 3 -0.28765908732027157 1109 4 -0.20238813616635554 1109 5 -0.2445553348260603 1109 6 -0.38969606402127255 1109 7 -0.30252165383790697 1109 8 -0.33934044775693195 1109 9 -0.4299625676256298 1109 10 -0.4097221497992507 1109 11 -0.43587595250053823 1109 12 -0.586474440382915 1109 13 -0.6200810355740174 1109 14 -0.7386644345902127 1109 15 -0.8920712703727531 1109 16 -0.7586833039297289 1109 17 -0.9486885365943749 1109 18 -0.8864954537170817 1109 19 -0.9754421033676124 1109 20 -0.9636387355890079 1109 21 -0.9890471327082437 1109 22 -1.0764465465655277 1109 23 -1.0038394196696245 1109 24 -1.1473469930486857 1109 25 -1.2387654117622233 1110 1 0.5713185229664504 1110 2 0.09994749809314385 1110 3 -0.2343003833730748 1110 4 -0.12265736077031164 1110 5 -0.08539760299302657 1110 6 -0.11732116899990785 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0.3434971784783052 2314 4 0.0803678486931858 2314 5 -0.0275868784251756 2314 6 -0.17660554552006147 2314 7 -0.36804016716821764 2314 8 -0.321800419032904 2314 9 -0.2627905079731008 2314 10 -0.3196471600145543 2314 11 -1.0464290524515363 2314 12 -0.6626830332592988 2314 13 -0.4392897821040833 2314 14 -0.3606002674537834 2314 15 -0.47359909211121476 2314 16 -0.5954488017458601 2314 17 -0.9711614787619425 2314 18 -1.8171639538874587 2314 19 -1.6535545962847635 2314 20 -2.027210403400403 2314 21 -1.4308157787284443 2314 22 -0.9517568227980178 2314 23 -0.7413768697261176 2314 24 -0.723238584314366 2314 25 -0.6438823218593015 2315 1 -2.6267427184947643 2315 2 -1.2897948886018966 2315 3 -0.5889603781889754 2315 4 -0.47044167358516376 2315 5 -0.36651302624947135 2315 6 -0.26333875921854855 2315 7 -0.30205036346738723 2315 8 -0.22379697450323022 2315 9 -0.26531609897873665 2315 10 -0.2467794969368171 2315 11 -0.464336596419507 2315 12 -0.33282162734636955 2315 13 -0.34032007004783793 2315 14 -0.37932876849808395 2315 15 -0.9946931331182398 2315 16 -0.8445373598304755 2315 17 -0.6471475358954635 2315 18 -0.3576695128612144 2315 19 -0.3246645159449173 2315 20 -0.3845919177578523 2315 21 -0.43495994461503074 2315 22 -0.32994948884736175 2315 23 -0.3364374344136536 2315 24 -0.23863871011521098 2315 25 -0.1378160983015988 2316 1 1.6734137407929204 2316 2 0.8626092544303852 2316 3 0.2670868155338191 2316 4 0.17018773098222084 2316 5 -0.02857680996865497 2316 6 -0.15807582371152762 2316 7 -0.036122542386308965 2316 8 -0.10095304324632082 2316 9 -0.1329080505005038 2316 10 -0.12318978848107065 2316 11 -0.3399330521820332 2316 12 -0.1858058830269229 2316 13 -0.20235294822870742 2316 14 -0.31475671664197336 2316 15 -0.24801155493175742 2316 16 -0.19305163040242945 2316 17 -0.1623375777929965 2316 18 -0.02855872918247826 2316 19 -0.19573318962046216 2316 20 -0.09744536212576804 2316 21 -0.11002216313770674 2316 22 -0.2544649751176422 2316 23 -0.1232304174077612 2316 24 -0.2959583729792499 2316 25 -0.2961398289863908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43478.0 3 1.0 4 331.0 5 1789.0 6 2237.0 7 1989.0 8 1850.0 9 1758.0 10 1742.0 11 1792.0 12 1720.0 13 1833.0 14 1814.0 15 1849.0 16 2007.0 17 2052.0 18 2259.0 19 2843.0 20 3367.0 21 4209.0 22 5205.0 23 6255.0 24 7470.0 25 8596.0 26 9682.0 27 10692.0 28 12722.0 29 15184.0 30 19650.0 31 25556.0 32 34136.0 33 46849.0 34 66770.0 35 96952.0 36 172350.0 37 292016.0 38 914975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79676572826072 19.43849525301238 12.19823310388415 24.56650591484275 2 14.411471479224286 20.030980244601114 42.3291760847892 23.228372191385404 3 18.71811650219795 25.045780376165528 29.663211367708307 26.572891753928214 4 12.167623103469872 16.831639654606004 38.74596357299739 32.25477366892673 5 13.074277729533954 37.9731442102193 35.801952419322504 13.150625640924238 6 31.356598957598425 37.20219548900251 18.448630180232364 12.992575373166698 7 27.7785868355613 31.62004204047426 22.633107560416008 17.968263563548437 8 24.86078992753659 37.48466422832705 20.72890860951458 16.925637234621785 9 26.8564889252045 15.089244137519001 20.50166056037323 37.55260637690328 10 15.593070161377096 28.560331406060257 34.45079305127659 21.395805381286053 11 33.99786748775343 21.927046558386582 25.652574003775268 18.42251195008472 12 24.189637773382984 24.10431625841548 31.99199932357718 19.71404664462436 13 29.39117780309999 22.15999670506185 25.707711528164857 22.741113963673307 14 20.94245466296349 22.490991896275844 27.65122687023169 28.915326570528975 15 22.65695502254973 31.850792565568987 23.31146326476999 22.180789147111295 16 21.79868861545771 27.602697994430685 28.678563415846593 21.920049974265012 17 21.470512530002267 28.64762654851292 28.165610308239597 21.716250613245215 18 21.35643290757246 26.724079321355887 30.897014702786162 21.022473068285493 19 23.912582149584214 26.2269453377969 27.69685055561496 22.16362195700393 20 24.045109786791347 26.89456769787658 28.435154818662628 20.62516769666944 21 22.948417752603422 26.28066638387588 27.273360833182885 23.497555030337818 22 23.63518493431304 28.17757555248293 27.322243728160796 20.864995785043238 23 22.11231759715568 27.861682704847148 28.311282551100252 21.71471714689692 24 23.564626239186914 26.31217644559669 28.808715213865373 21.31448210135102 25 22.717203468063804 27.2363089939503 28.571884139089832 21.47460339889607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 845.0 1 845.0 2 1243.5 3 1642.0 4 1642.0 5 1642.0 6 2682.5 7 3723.0 8 3723.0 9 3723.0 10 4305.0 11 4887.0 12 4887.0 13 4887.0 14 6735.5 15 8584.0 16 8584.0 17 8584.0 18 15138.5 19 21693.0 20 21693.0 21 21693.0 22 34515.0 23 47337.0 24 47337.0 25 47337.0 26 69846.5 27 92356.0 28 92356.0 29 92356.0 30 119300.0 31 146244.0 32 146244.0 33 146244.0 34 162269.5 35 178295.0 36 178295.0 37 178295.0 38 188650.0 39 199005.0 40 199005.0 41 199005.0 42 208422.0 43 217839.0 44 217839.0 45 217839.0 46 225300.5 47 232762.0 48 232762.0 49 232762.0 50 225014.5 51 217267.0 52 217267.0 53 217267.0 54 193838.5 55 170410.0 56 170410.0 57 170410.0 58 147173.0 59 123936.0 60 123936.0 61 123936.0 62 102550.5 63 81165.0 64 81165.0 65 81165.0 66 62870.0 67 44575.0 68 44575.0 69 44575.0 70 32556.5 71 20538.0 72 20538.0 73 20538.0 74 14386.0 75 8234.0 76 8234.0 77 8234.0 78 5606.5 79 2979.0 80 2979.0 81 2979.0 82 2029.5 83 1080.0 84 1080.0 85 1080.0 86 716.0 87 352.0 88 352.0 89 352.0 90 246.5 91 141.0 92 141.0 93 141.0 94 98.5 95 56.0 96 56.0 97 56.0 98 45.5 99 35.0 100 35.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3507705451319292 2 0.2992913394451199 3 0.3218545657674235 4 0.31117536884303226 5 0.29392435842670783 6 0.2524124032026638 7 0.3452940338886516 8 0.3225117471166168 9 0.26878717182006373 10 0.24140461560367582 11 0.25487683326213867 12 0.25378153101348316 13 0.274099387726043 14 0.26517267439950054 15 0.35301591474167293 16 0.4076714969495832 17 0.426291635176727 18 0.11544485700829145 19 0.1273288864062038 20 0.19129453772768595 21 0.20082366729098894 22 0.14846821980525526 23 0.2631463652394878 24 0.35821860042278664 25 0.39502075597761205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1825980.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.88221640421351 #Duplication Level Percentage of deduplicated Percentage of total 1 82.16265516632852 54.95220483179154 2 11.762566480916384 15.734130336911957 3 3.1240854988535256 6.268372871987604 4 1.1750381413055062 3.143566209999987 5 0.5423103735789829 1.8135459881979705 6 0.3004264827735983 1.2055913420652316 7 0.1799496574048988 0.8424802349892966 8 0.13331764261606965 0.7133263539158055 9 0.08594748623194574 0.517352253620684 >10 0.47259296110546595 5.661522535311399 >50 0.03231102550045825 1.5175140007741683 >100 0.02419202798418702 3.2887852423894235 >500 0.00296167847174827 1.4465606616894027 >1k 0.0015631080823115863 2.5567093079520644 >5k 8.226884643745191E-5 0.33833782840350457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6149 0.3367506763491385 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4691 0.2569031424221514 No Hit GTCCTACAGTGGACATTTCTAAATT 4681 0.25635549129782365 No Hit CTGTAGGACGTGGAATATGGCAAGA 4239 0.2321493116025367 No Hit CTTTAGGACGTGAAATATGGCGAGG 4146 0.22705615614628857 No Hit GGTATCAACGCAGAGTACTTTTTTT 3513 0.19238983997634146 No Hit TATCAACGCAGAGTACTTTTTTTTT 3405 0.1864752078336017 No Hit GTCCTACAGTGTGCATTTCTCATTT 3008 0.16473345819778967 No Hit CTGTAGGACCTGGAATATGGCGAGA 2098 0.11489720588396368 No Hit CTGAAGGACCTGGAATATGGCGAGA 2000 0.10953022486555165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 5.4765112432775826E-5 13 0.0 0.0 0.0 0.0 1.0953022486555165E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTCG 55 1.9792048E-4 13.802671 9 GTCGTAC 50 0.0015012242 13.298228 1 TTCGATT 50 0.0015021581 13.297131 12 GTTCGAT 50 0.001504651 13.294205 11 GGTATCA 1040 0.0 13.243428 1 GCACTCG 60 4.086773E-4 12.668814 13 GTGTAGG 340 0.0 12.571855 1 TAGGACC 855 0.0 12.222276 4 ATAGGAC 190 0.0 11.500367 3 GGCGAGG 1170 0.0 11.454756 19 GTATCAA 2500 0.0 11.398481 1 CGATTCC 60 0.005842504 11.091927 14 AGCTCGT 60 0.0059014363 11.076981 10 CACCTTT 620 0.0 10.887466 14 AATCCCG 140 8.283678E-9 10.862868 19 GTCCTAC 2565 0.0 10.665246 1 CAAGTCG 235 0.0 10.510106 6 TGGCGAG 2595 0.0 10.508234 18 GGACCTG 1575 0.0 10.374077 6 TCCAACG 110 6.0356433E-6 10.365108 18 >>END_MODULE