FastQCFastQC Report
Thu 26 May 2016
SRR1512335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825980
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69030.37804357112345155No Hit
GTCCTACAGTGGACATTTCTAAATT48540.26582985574869383No Hit
GTCCTAAAGTGTGTATTTCTCATTT47220.25860086090756745No Hit
CTGTAGGACGTGGAATATGGCAAGA45340.24830501977020558No Hit
CTTTAGGACGTGAAATATGGCGAGG43760.23965213200582702No Hit
TATCAACGCAGAGTACTTTTTTTTT42980.2353804532360705No Hit
GGTATCAACGCAGAGTACTTTTTTT40370.22108675889111598No Hit
GTCCTACAGTGTGCATTTCTCATTT30360.1662668813459074No Hit
CTGTAGGACCTGGAATATGGCGAGA23430.12831465842999376No Hit
CTGAAGGACCTGGAATATGGCGAGA21140.1157734476828881No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19620.10744915059310617No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18280.1001106255271142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.013.6015291
TAGGACC8700.013.3264324
CCGGTTT1207.421477E-1012.66478412
CCAACGA1501.8189894E-1212.65819619
TCCAACG1900.012.4916418
AGGACCT16000.011.4633265
GTATCAA25400.011.4591941
CTAGCAC1501.7462298E-1011.4039313
CGGTTTC1251.8391802E-811.39705813
ATGTCGA1553.1650416E-1011.02487917
GGACCTG15300.010.9957946
CACCTTT6900.010.87043114
GACAGTC2100.010.8626737
TTAGTAC1158.778752E-710.7428343
CTACACT3450.010.7428344
TTAGGAC16000.010.631793
GACGTGA13350.010.60839757
GTCCTAC25800.010.5810451
GCCGGTT1355.700167E-810.55398811
TGTCGAG1355.743823E-810.54849618
Produced by FastQC (version 0.11.5)