FastQCFastQC Report
Thu 26 May 2016
SRR1512334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704582
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59530.3492351790644275No Hit
GTCCTAAAGTGTGTATTTCTCATTT46250.2713275160713888No Hit
GTCCTACAGTGGACATTTCTAAATT43010.2523199235941715No Hit
CTGTAGGACGTGGAATATGGCAAGA41210.24176014999571743No Hit
CTTTAGGACGTGAAATATGGCGAGG39020.22891242545093168No Hit
GGTATCAACGCAGAGTACTTTTTTT35710.20949417511155224No Hit
TATCAACGCAGAGTACTTTTTTTTT33690.19764376251773164No Hit
GTCCTACAGTGTGCATTTCTCATTT25970.15235406686213981No Hit
CTGAAGGACCTGGAATATGGCGAGA20100.11791747184940354No Hit
ATTTAGAAATGTCCACTGTAGGACG17180.10078717245635586No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17110.10037651459419375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.015.0892191
TAGGACC7650.012.9185414
ATTAACC1550.012.8744643
TCCAACG1251.4078978E-912.161509518
GGACCGT550.003063760512.093486
AATGTCC6700.010.8954858
TGGACCG700.001488513310.8597735
GTACTAG1053.4635104E-610.8578511
ATTTAGA6850.010.8182231
AAATGTC6500.010.8176857
GCCTTAC1158.80902E-710.739831
GTCCTAT1951.8189894E-1210.7186471
TAGAAAT7300.010.6741324
GTCCTAA12750.010.6555971
TGTAGGA26850.010.5820182
GAAATGT7200.010.55786
AGGACCT15700.010.5312135
TTTAGAA7400.010.5298872
CTAGGAC2450.010.4722333
CTAGACT2001.8189894E-1210.4528384
Produced by FastQC (version 0.11.5)