FastQCFastQC Report
Thu 26 May 2016
SRR1512334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704582
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69450.4074312646736854No Hit
GTCCTAAAGTGTGTATTTCTCATTT45570.2673382682675283No Hit
CTGTAGGACGTGGAATATGGCAAGA45070.26440499782351334No Hit
GTCCTACAGTGGACATTTCTAAATT44770.26264503555710433No Hit
TATCAACGCAGAGTACTTTTTTTTT43730.2565438330335531No Hit
GGTATCAACGCAGAGTACTTTTTTT41390.24281612735556282No Hit
CTTTAGGACGTGAAATATGGCGAGG41360.2426401311289219No Hit
GTCCTACAGTGTGCATTTCTCATTT27500.16132987442082575No Hit
CTGAAGGACCTGGAATATGGCGAGA21810.1279492567679349No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21380.125426644186082No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18850.11058429573936601No Hit
ATTTAGAAATGTCCACTGTAGGACG18450.10823767938415399No Hit
TTTCTAAATTTTCCACCTTTTTCAG18160.10653638252662531No Hit
CTGTAGGACCTGGAATATGGCGAGA17600.10325111962932848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA250.00601757719.0080457
CCAACGA551.1352278E-515.53561319
TCCAACG1153.8198777E-1013.20902518
GGTATCA12200.013.1002021
GCCGGTT853.9524657E-612.29174411
TAGGACC7650.012.1740174
TCGAACT1251.8490027E-811.39278419
GGCGAGG9300.011.22944619
CCGTTTC951.36321105E-510.99787613
GTATCAA26350.010.9393261
TAAGACA1951.8189894E-1210.7215444
GTCTTAC2050.010.6733931
TGGCGAA1002.4155659E-510.44338518
AATCCCG1659.385985E-1010.35707719
GTATAAT2300.010.3404821
GTATTAT1201.496559E-610.3060121
TAGGTAG1201.5170081E-610.2951185
ATAGCCC1856.002665E-1110.2734133
CTAAGAC2053.6379788E-1210.1982413
GCCTCGA1409.763971E-810.17302316
Produced by FastQC (version 0.11.5)