FastQCFastQC Report
Thu 26 May 2016
SRR1512333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603493
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86210.3311320598903089No Hit
GTCCTAAAGTGTGTATTTCTCATTT78830.302785526982404No Hit
CTGTAGGACGTGGAATATGGCAAGA77650.2982531545120344No Hit
GTATCAACGCAGAGTACTTTTTTTT76210.2927221237007359No Hit
CTTTAGGACGTGAAATATGGCGAGG66740.25634791412921026No Hit
GGTATCAACGCAGAGTACTTTTTTT42360.16270448969903126No Hit
TATCAACGCAGAGTACTTTTTTTTT42230.16220516052856682No Hit
GTCCTACAGTGTGCATTTCTCATTT41400.15901713582483226No Hit
CTGAAGGACCTGGAATATGGCGAGA35290.13554866481300315No Hit
GTCCTTCAGTGTGCATTTCTCATTT29860.114692069462065No Hit
CTGTAGGACCTGGAATATGGCGAGA29070.11165768450308873No Hit
ATTTAGAAATGTCCACTGTAGGACG28210.10835442999078546No Hit
TTTCTAAATTTTCCACCTTTTTCAG27780.10670280273463381No Hit
GAATATGGCAAGAAAACTGAAAATC26600.10217043026426421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG551.9572652E-413.8212425
GGTATCA15850.013.30741
TAGGACC12100.013.1144764
TATGTCG2800.012.22478116
TGTCGAG2800.011.87671418
ATGTCGA2900.011.80323617
CTAGGAC3750.011.4027463
GTCGAGG2700.011.26479819
ATTTAGA10900.011.24430751
ATGTCCA11250.011.1306539
TTTAGAA12800.010.6900732
GTGTAGG4550.010.6494771
GTCCTAA23150.010.6296341
GTCCTAC42800.010.6109341
GTATCAA33950.010.5224671
TGTCCAC11850.010.4930910
CACCTTT9650.010.44258614
GCGCCAC1553.6579877E-910.42065813
AATGTCC11300.010.4061928
GGCGAGG18850.010.38707119
Produced by FastQC (version 0.11.5)