FastQCFastQC Report
Thu 26 May 2016
SRR1512333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603493
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87560.33631740127590126No Hit
GTCCTACAGTGGACATTTCTAAATT84050.32283551367336116No Hit
CTGTAGGACGTGGAATATGGCAAGA82570.31715084311730435No Hit
GTCCTAAAGTGTGTATTTCTCATTT79130.30393782506809125No Hit
CTTTAGGACGTGAAATATGGCGAGG72240.2774733790334754No Hit
TATCAACGCAGAGTACTTTTTTTTT54280.20848913363700228No Hit
GGTATCAACGCAGAGTACTTTTTTT51710.19861778003628203No Hit
GTCCTACAGTGTGCATTTCTCATTT41460.1592475954419697No Hit
CTGAAGGACCTGGAATATGGCGAGA39440.15148878833167595No Hit
CTGTAGGACCTGGAATATGGCGAGA31380.12053037976288009No Hit
ATTTAGAAATGTCCACTGTAGGACG30310.11642051659059578No Hit
TTTCTAAATTTTCCACCTTTTTCAG29760.11430797010016927No Hit
GTCCTTCAGTGTGCATTTCTCATTT29060.11161927456689916No Hit
GAATATGGCAAGAAAACTGAAAATC27870.10704849216033997No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26590.10213202032807463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.013.4195171
TAGGACC13850.013.10438254
TAGTACG550.003063616812.0939554
GGCGAGG18500.011.90621519
GTCCTAT4100.011.6016661
GGACCGT951.3549545E-511.0044636
CACCTTT11200.010.93589114
AGGACCT26650.010.376375
GTATCAA33000.010.3493891
TAGAAAT10500.010.2263854
TCCTATA4750.010.20237352
TGTAGGA41850.010.172022
CTATAGT4400.010.1502844
ACCTTTT11800.010.13785415
ATACACG750.002643068310.1358864
TGGCGAG42850.010.12545318
ATGCCGA856.5715675E-410.0629056
GTGGTAT5500.010.0322771
GGACCTG25900.010.0175766
CCACCTT12150.010.00671213
Produced by FastQC (version 0.11.5)