FastQCFastQC Report
Thu 26 May 2016
SRR1512332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306884
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78530.3404159030102944No Hit
GTATCAACGCAGAGTACTTTTTTTT74580.3232932388451262No Hit
GTCCTAAAGTGTGTATTTCTCATTT70910.3073843331524255No Hit
CTGTAGGACGTGGAATATGGCAAGA70130.3040031488362657No Hit
CTTTAGGACGTGAAATATGGCGAGG61580.26694016690912936No Hit
TATCAACGCAGAGTACTTTTTTTTT41550.18011308761082048No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.17707869142965144No Hit
GTCCTACAGTGTGCATTTCTCATTT38850.1684089880548827No Hit
CTGAAGGACCTGGAATATGGCGAGA32170.13945217878315513No Hit
CTGTAGGACCTGGAATATGGCGAGA27470.11907837585244858No Hit
ATTTAGAAATGTCCACTGTAGGACG27460.11903502733557475No Hit
GTCCTTCAGTGTGCATTTCTCATTT26230.11370315976009196No Hit
TTTCTAAATTTTCCACCTTTTTCAG24380.10568368413843088No Hit
GAATATGGCAAGAAAACTGAAAATC24340.10551029007093553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA350.002170806816.285517
TAGACCG400.00528132214.2495115
GGTATCA13300.013.3552291
TAGGACC12450.013.3529554
AATGTCC9200.012.88919458
AAATGTC9600.012.1717217
ATGTCCA10350.011.9171599
AGTCGTC2750.011.72878
GGCGAGG17200.011.38396419
GGACCTG23550.011.2541456
CAAGTCG2800.011.1957996
TGTCCAC10650.011.14281210
AGGACCT24700.011.1150035
TAGAAAT10700.011.0977494
TAAGACG600.005878609611.0829524
GTCCGCT600.005898655411.07788511
CCACCTT9900.011.03737813
AAGTCGT3050.010.9015027
GAAATGT10650.010.8820186
TGTAGGA41850.010.827822