FastQCFastQC Report
Thu 26 May 2016
SRR1512332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306884
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88000.3814669484898244No Hit
CTGTAGGACGTGGAATATGGCAAGA77010.3338269284454702No Hit
GTCCTACAGTGGACATTTCTAAATT76600.33204963925364256No Hit
GTCCTAAAGTGTGTATTTCTCATTT73680.3193918723264802No Hit
CTTTAGGACGTGAAATATGGCGAGG67130.2909985937741126No Hit
TATCAACGCAGAGTACTTTTTTTTT54480.23616271992870033No Hit
GGTATCAACGCAGAGTACTTTTTTT52190.22623590956459017No Hit
GTCCTACAGTGTGCATTTCTCATTT40430.17525805372095No Hit
CTGAAGGACCTGGAATATGGCGAGA34860.15111292982221905No Hit
CTGTAGGACCTGGAATATGGCGAGA30310.13138935464462018No Hit
ATTTAGAAATGTCCACTGTAGGACG28200.12224281758423916No Hit
TTTCTAAATTTTCCACCTTTTTCAG28060.12163593834800537No Hit
GTCCTTCAGTGTGCATTTCTCATTT26560.11513366081692882No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25770.11170912798389517No Hit
GAATATGGCAAGAAAACTGAAAATC24640.10681074557715083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC400.005259163714.2585478
GGTATCA14200.014.1986941
TAGGACC14350.013.9074564
CCAACGA1155.3842086E-912.38260919
ATTTAGA9650.012.2206771
GTCGAGG1251.4279067E-912.15146719
AAACCGT550.003055466612.0981616
GCGTGCG1103.7955033E-812.09579859
CGTGCGC1201.00390025E-811.87361810
TATGTCG1603.6379788E-1211.86666816
GGCGAGG19350.011.82373919
TTTAGAA11150.011.67635252
CACCGTC907.4151685E-611.6180747
TAGAAAT10400.011.513774
CACCTTT10100.011.46794114
GAAATGT9450.011.3666016
GCCGGTT1604.5474735E-1111.27993711
AAATGTC9350.011.0815097
TGGCGAG42550.010.97701618
AGGACCT26850.010.9378575
Produced by FastQC (version 0.11.5)