FastQCFastQC Report
Thu 26 May 2016
SRR1512331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865404
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68370.3665157788875761No Hit
GGTATCAACGCAGAGTACTTTTTTT38050.20397726176206335No Hit
GTCCTAAAGTGTGTATTTCTCATTT38040.20392365407171853No Hit
TATCAACGCAGAGTACTTTTTTTTT38030.2038700463813737No Hit
GTCCTACAGTGGACATTTCTAAATT36740.1969546543268911No Hit
CTGTAGGACGTGGAATATGGCAAGA36240.1942742698096498No Hit
CTTTAGGACGTGAAATATGGCGAGG31020.1662910554496506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.013.7548371
GGTCTAA707.2688345E-613.570621
TAGGACC6550.012.618934
GTATCAA27250.010.9809971
GGCGAGG8450.010.9143819
CGAATGG803.7426027E-410.69637419
TAACCTA803.7697385E-410.6880155
ACTGTCG803.840725E-410.6664588
AATGTCC5450.010.4381248
GATATAC3050.010.2780761
CTAGGAC3250.010.2318143
GGACCTG12150.010.1650996
TTACACC1701.5570549E-910.0593084
TTTAGGA13500.09.9936122
CCTACAC2105.456968E-129.9533983
TTAGGAC12800.09.9463273
TGGCGAG20100.09.83556118
AGGACCT13650.09.813665
GTCTTAT1651.0229996E-89.7872951
AATGCCG1201.6907783E-59.50455618
Produced by FastQC (version 0.11.5)