FastQCFastQC Report
Thu 26 May 2016
SRR1512331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865404
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82490.44220983765447053No Hit
TATCAACGCAGAGTACTTTTTTTTT50810.27238067464206145No Hit
GGTATCAACGCAGAGTACTTTTTTT48990.26262407499930307No Hit
CTGTAGGACGTGGAATATGGCAAGA38690.2074081539441322No Hit
GTCCTAAAGTGTGTATTTCTCATTT37820.20274428488413235No Hit
GTCCTACAGTGGACATTTCTAAATT37710.20215460029033924No Hit
CTTTAGGACGTGAAATATGGCGAGG34270.18371355481171908No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23630.12667497228482408No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20260.1086091806386177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.006024310419.004485
CTACACG359.9230216E-519.004484
GGTATCA14000.014.4053381
TAGGACC5200.011.3295954
AAGTCGT1658.0035534E-1110.9443227
AGTCGTC1752.0008883E-1110.8620358
GTGGTAT4050.010.5699561
GGCGAGG7750.010.41343119
CTTATAC1752.3464963E-1010.3161643
GTCCTAA11750.010.2820921
ATTTAGA5600.010.1924561
AGACCGT750.00263811910.1378986
CGCCCTA1054.1360083E-59.94565616
GTATCAA29100.09.9052461
CACCTTT5100.09.86772814
GATATAC3200.09.810241
CAAGTCG1752.5520421E-99.7758316
CAAGACT1951.6189006E-109.7458884
TAGAAAT7100.09.6360754
GAAATGT6500.09.504286
Produced by FastQC (version 0.11.5)