FastQCFastQC Report
Thu 26 May 2016
SRR1512330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699213
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67360.39641881270917767No Hit
GTCCTAAAGTGTGTATTTCTCATTT63480.3735847124521764No Hit
CTGTAGGACGTGGAATATGGCAAGA62150.36575755952902905No Hit
GTATCAACGCAGAGTACTTTTTTTT57370.3376268896247851No Hit
CTTTAGGACGTGAAATATGGCGAGG53820.31673486490510605No Hit
GTCCTACAGTGTGCATTTCTCATTT35660.20986185957852255No Hit
TATCAACGCAGAGTACTTTTTTTTT32110.18896983485884347No Hit
GGTATCAACGCAGAGTACTTTTTTT30620.1802010695539641No Hit
CTGAAGGACCTGGAATATGGCGAGA26560.15630765536751426No Hit
ATTTAGAAATGTCCACTGTAGGACG23350.13741655695901572No Hit
GTCCTTCAGTGTGCATTTCTCATTT22920.13488597368311095No Hit
CTGTAGGACCTGGAATATGGCGAGA22360.1315903303470489No Hit
TTTCTAAATTTTCCACCTTTTTCAG21470.12635261147366458No Hit
GAATATGGCAAGAAAACTGAAAATC21310.12541099909193254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATG350.002168753416.2874622
CGGTAGC456.7633286E-414.7776171
GGTATCA8950.013.9048771
TATGTCG1700.013.42073316
TGTCGAG1900.013.0010118
GCGCCAC905.3930853E-712.669147513
TAGGACC9600.012.5690564
TCCAACG1457.2759576E-1212.44924418
GTGTAGG3900.012.4229421
AATGTCC7850.011.96556958
CCAACGA1209.9717E-911.87837819
TAGAAAT8050.011.8025084
ATGTCGA1950.011.70012617
CGTGCGC658.062652E-411.68562410
AAATGTC7450.011.6045867
ATTAGAC752.0719603E-411.4012223
CCTACAC3000.011.4012223
TTTAGAA8450.011.243812
CACCTTT7650.011.18361414
GAAATGT8050.011.0937036
Produced by FastQC (version 0.11.5)