FastQCFastQC Report
Thu 26 May 2016
SRR1512330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699213
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71030.4180170467151557No Hit
CTGTAGGACGTGGAATATGGCAAGA65130.38329509013878776No Hit
GTCCTACAGTGGACATTTCTAAATT61130.35975478059548743No Hit
GTCCTAAAGTGTGTATTTCTCATTT58620.34498323635706646No Hit
CTTTAGGACGTGAAATATGGCGAGG56710.3337427385501406No Hit
TATCAACGCAGAGTACTTTTTTTTT45150.26571124397000256No Hit
GGTATCAACGCAGAGTACTTTTTTT40940.24093506817567897No Hit
GTCCTACAGTGTGCATTTCTCATTT30890.18179004044813687No Hit
CTGAAGGACCTGGAATATGGCGAGA30210.17778818782577582No Hit
ATTTAGAAATGTCCACTGTAGGACG24990.14706808387176887No Hit
CTGTAGGACCTGGAATATGGCGAGA23660.1392409309486215No Hit
TTTCTAAATTTTCCACCTTTTTCAG23630.13906437862704676No Hit
GAATATGGCAAGAAAACTGAAAATC22960.13512137677854397No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22220.1307664195130334No Hit
GTCCTTCAGTGTGCATTTCTCATTT20840.12264501272059476No Hit
GGAATATGGCGAGAAAACTGAAAAT19090.11234612729540086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.014.3789651
CCAACGA954.9294613E-913.9944519
ATACGGC903.6585334E-813.71678215
CTTTACG500.001491175713.3100021
TAGGACC11400.012.5024024
AATACGG1001.4485158E-712.34619214
TTAGTAC1251.4078978E-912.1619793
TCCAACG1251.4206307E-912.15482118
GTATCGC550.003060180712.0953696
GTCGAGA550.003077774812.08611519
TAGAAAT8800.011.8772834
CACCTTT8150.011.65284914
AAATGTC8350.011.6090717
GGCGAGG16150.011.34843919
AATGTCC8800.011.3394098
CGCCCTA1354.7602953E-911.25446316
GTCCTAT2200.011.2357171
TAGTACG855.312894E-511.1786184
ACGGCAA600.00589470111.07861317
ACCTTTT8500.011.06031915
Produced by FastQC (version 0.11.5)