FastQCFastQC Report
Thu 26 May 2016
SRR1512329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938274
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85640.4418363967117136No Hit
GTCCTACAGTGGACATTTCTAAATT50420.2601283409879099No Hit
TATCAACGCAGAGTACTTTTTTTTT49930.2576003186340012No Hit
GGTATCAACGCAGAGTACTTTTTTT49730.25656847277526296No Hit
GTCCTAAAGTGTGTATTTCTCATTT48680.2511512820168872No Hit
CTGTAGGACGTGGAATATGGCAAGA44160.22783156560940301No Hit
CTTTAGGACGTGAAATATGGCGAGG42630.21993794479005546No Hit
GTCCTACAGTGTGCATTTCTCATTT28610.14760555009250498No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23610.12180940362404903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23020.11876545834077121No Hit
CTGTAGGACCTGGAATATGGCGAGA20700.10679604637940766No Hit
CTGAAGGACCTGGAATATGGCGAGA20530.10591897739948015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.014.6592561
CGAATTT551.9432609E-413.83260615
TCGAAAC701.0795395E-412.22830616
ACTAGAC701.0908437E-412.2146683
TAGGACC8150.011.8899444
GGCGAGG12250.011.33015119
GTATACG600.00587628911.0833931
CACCTTT6350.011.08242314
GTATCAA31900.010.8699661
GGACCTG14800.010.655746
GTGTAGG3300.010.6515731
AGGACCT16100.010.5624445
TAGAAAT6700.010.49286654
ACCTTTT6950.010.39933415
GTCCTAC24400.010.3566141
GACGTGA15400.010.3022717
TCGAACT1201.5107034E-610.29866719
TGTAGAA5450.010.2847262
GTCCTAA15100.010.2550231
ACGTGAA17350.010.1655118
Produced by FastQC (version 0.11.5)