FastQCFastQC Report
Thu 26 May 2016
SRR1512329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938274
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102350.5280471182092935No Hit
TATCAACGCAGAGTACTTTTTTTTT63990.3301390825032993No Hit
GGTATCAACGCAGAGTACTTTTTTT58870.30372382851960045No Hit
GTCCTAAAGTGTGTATTTCTCATTT50810.26214044041244944No Hit
GTCCTACAGTGGACATTTCTAAATT50630.261211779139585No Hit
CTGTAGGACGTGGAATATGGCAAGA45700.23577677872168742No Hit
CTTTAGGACGTGAAATATGGCGAGG43970.22685131204360168No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29020.14972083410291837No Hit
GTCCTACAGTGTGCATTTCTCATTT28280.1459030044255869No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26660.13754505296980715No Hit
CTGAAGGACCTGGAATATGGCGAGA22920.11824953541140211No Hit
CTGTAGGACCTGGAATATGGCGAGA22260.11484444407756592No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20770.10715719242996603No Hit
TTTCTAAATTTTCCACCTTTTTCAG20220.10431961631843589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.014.1699831
TAGGACC7300.012.6266334
TCCAACG1551.8189894E-1212.2511918
GCCGGTT1104.974536E-711.2264811
ACCTTTT7500.011.14070315
CCGTAGG600.00582450511.0965851
AATGTCC7450.010.9713328
GGCGAGG12550.010.894567519
AGGACCT16750.010.7222445
GTATCAA32450.010.6984461
TAGAAAT7400.010.658234
TATACTG3600.010.5583525
CACCTTT7600.010.49465314
CGGGGGA2001.8189894E-1210.4441417
TGGCGAG26250.010.41701218
GGACCTG16600.010.30553156
AAATGTC7750.010.3013647
TGTCCAC8300.010.30047710
ACCGGGG1951.4551915E-1110.22529615
AGGCGTG1502.428169E-810.1378517
Produced by FastQC (version 0.11.5)