FastQCFastQC Report
Thu 26 May 2016
SRR1512328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260449
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72620.321263607362962No Hit
GTATCAACGCAGAGTACTTTTTTTT67600.2990556300982681No Hit
CTGTAGGACGTGGAATATGGCAAGA64300.28445676058163666No Hit
GTCCTAAAGTGTGTATTTCTCATTT64090.28352774161239647No Hit
CTTTAGGACGTGAAATATGGCGAGG54320.2403062400434604No Hit
TATCAACGCAGAGTACTTTTTTTTT38110.1685948234178254No Hit
GGTATCAACGCAGAGTACTTTTTTT37050.16390548957308926No Hit
GTCCTACAGTGTGCATTTCTCATTT33470.14806792809747088No Hit
CTGAAGGACCTGGAATATGGCGAGA29150.1289566807302443No Hit
CTGTAGGACCTGGAATATGGCGAGA25460.11263249027073825No Hit
GTCCTTCAGTGTGCATTTCTCATTT24110.10666022546847993No Hit
ATTTAGAAATGTCCACTGTAGGACG23720.1049349045256053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.013.6853381
TAGGACC10900.012.2912484
GCGAATC550.003047137812.102550515
ACCGTAT658.1490376E-411.6710998
AAGTCGT3200.011.2833237
TATGTCG1701.0913936E-1111.18797816
ACTCCCG600.00586998411.08512318
AAATGTC8300.010.9899717
TCCAACG1658.0035534E-1110.9411618
GTATCAA27900.010.9318731
CACCTTT9150.010.91213514
CCACCTT8950.010.82784313
AGTCGTC3450.010.7196518
GGCGAGG14950.010.61729519
ACCTTTT8700.010.60215215
TAATACT2700.010.5574954
GGACCTG21050.010.51713856
TGTAGGA39250.010.506392
TAGAAAT9700.010.383354
CCAACGA1106.0123148E-610.36876919
Produced by FastQC (version 0.11.5)