FastQCFastQC Report
Thu 26 May 2016
SRR1512328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260449
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82620.3655026058982087No Hit
GTCCTACAGTGGACATTTCTAAATT71810.317680248481607No Hit
CTGTAGGACGTGGAATATGGCAAGA69290.3065320208507248No Hit
GTCCTAAAGTGTGTATTTCTCATTT65420.28941152841758433No Hit
CTTTAGGACGTGAAATATGGCGAGG60270.2666284441719322No Hit
TATCAACGCAGAGTACTTTTTTTTT48010.21239143196771967No Hit
GGTATCAACGCAGAGTACTTTTTTT45370.20071233635441452No Hit
GTCCTACAGTGTGCATTTCTCATTT35660.15775626877668994No Hit
CTGAAGGACCTGGAATATGGCGAGA31410.13895469439921007No Hit
CTGTAGGACCTGGAATATGGCGAGA27490.12161300697339335No Hit
ATTTAGAAATGTCCACTGTAGGACG25000.11059749633811689No Hit
GTCCTTCAGTGTGCATTTCTCATTT24710.10931456538059474No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24630.10896065339231276No Hit
TTTCTAAATTTTCCACCTTTTTCAG24220.10714685445236764No Hit
GAATATGGCAAGAAAACTGAAAATC23160.10245752060763148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA350.0021663416.2905169
TAGTACG456.747775E-414.7824624
GGTATCA13650.014.3555271
TACGGCG602.5836247E-514.24158716
TAGGACC10450.012.9131834
GGACCGT701.08675034E-412.2197816
TAGAAAT10650.011.867614
GGCGAGG17250.011.55813519
AATGTCC9700.011.4641728
TTTAGAA11350.011.302642
GTCCTAT3000.011.0976581
ATTTAGA10600.010.85835551
TCCAACG1502.1555024E-910.76007218
AAGGCGT1605.4023985E-1010.6923086
TGTCCAC10850.010.59266810
GTCCTAG4050.010.56921
AAATGTC10350.010.5605387
GTATCAA31400.010.4816831
GAAATGT11150.010.31405456
TTAGAAA11450.010.2910153
Produced by FastQC (version 0.11.5)