FastQCFastQC Report
Thu 26 May 2016
SRR1512327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859970
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70410.37855449281440023No Hit
TATCAACGCAGAGTACTTTTTTTTT39810.2140357102533912No Hit
GGTATCAACGCAGAGTACTTTTTTT39720.21355183148115292No Hit
GTCCTACAGTGGACATTTCTAAATT28640.15398097818782025No Hit
CTGTAGGACGTGGAATATGGCAAGA26080.140217315333043No Hit
GTCCTAAAGTGTGTATTTCTCATTT26060.14010978671699006No Hit
CTTTAGGACGTGAAATATGGCGAGG22860.12290520814851852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19220.10333500002688216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT400.005273434314.2525136
GGTATCA12550.013.6264291
AACCGTC500.001497744313.3023457
TCCAACG1700.012.8548618
TAGGACC6200.011.80046754
GGCGAGG7400.011.43263319
CCAACGA1451.20599E-911.14471519
GTATCAA28800.010.8531711
TAACCTA1553.6525307E-910.4211925
GTATAGA2750.010.3643441
TAGGACA6000.010.2934824
TGGCGAG14300.010.23229718
GTAGGAC16750.010.0973033
TGTAGAA6500.010.0863932
GTCCTAA9900.010.0764471
TGTAGGA17450.010.0734092
GAACAGT3400.010.0605976
TTTAGAA6450.010.017272
TCCTAAA10750.09.9878072
AGGACGT17600.09.933575
Produced by FastQC (version 0.11.5)