FastQCFastQC Report
Thu 26 May 2016
SRR1512327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859970
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83840.4507599584939542No Hit
GGTATCAACGCAGAGTACTTTTTTT50120.2694667118286854No Hit
TATCAACGCAGAGTACTTTTTTTTT50110.2694129475206589No Hit
GTCCTACAGTGGACATTTCTAAATT29190.15693801512927627No Hit
CTGTAGGACGTGGAATATGGCAAGA27010.14521739597950506No Hit
GTCCTAAAGTGTGTATTTCTCATTT26180.14075495841330773No Hit
CTTTAGGACGTGAAATATGGCGAGG25380.13645381377118984No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23490.12629235955418636No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21250.11424915455625628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.014.1662081
TAGGACC4550.011.9037954
CGAATGG752.0854425E-411.39338719
ACGGTAC1451.2132659E-911.1398553
GCCGGTT1451.2169039E-911.13865711
CTGGTCG600.005859444411.0876359
GTATCAA28600.011.0364091
TACACCG700.0014884910.8598965
TAGAACG700.001488824110.8596034
TGTAGGA16750.010.6078012
CGTCGGT909.572613E-510.54943219
GCCCTAT750.002631241210.1410861
ATAGGAC2550.010.0605613
AGGACGT16500.010.0207215
CTGCGAT951.6377914E-410.00388059
TCCAACG1901.0004442E-109.99419818
GTAGGAC15700.09.9858023
GGCGAGG7800.09.98138619
GTATAAG2500.09.8875591
GGACGTG16450.09.8792126
Produced by FastQC (version 0.11.5)