FastQCFastQC Report
Thu 26 May 2016
SRR1512326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537961
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76180.30016221683469524No Hit
GTCCTACAGTGGACATTTCTAAATT50490.19893922719852666No Hit
GTCCTAAAGTGTGTATTTCTCATTT47980.18904939831620737No Hit
TATCAACGCAGAGTACTTTTTTTTT45250.1782927318426091No Hit
CTGTAGGACGTGGAATATGGCAAGA44650.17592862932093914No Hit
GGTATCAACGCAGAGTACTTTTTTT44060.17360392850796366No Hit
CTTTAGGACGTGAAATATGGCGAGG41780.16462033892561784No Hit
GTCCTACAGTGTGCATTTCTCATTT29870.11769290387046924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.013.4165621
CCGGTTT1201.0080839E-811.87049712
GGACGGG1052.7188798E-711.7630336
TAACGTG658.017352E-411.6938925
TAGGACC10450.011.5470184
AACCGTG600.005872326411.0846167
TCATTCG600.005935794711.0686179
GTATAAG2750.011.0553871
CGGTTTC1303.25781E-810.96410913
AGGACCT17600.010.958875
GGCGAGG11550.010.53185919
GTATCAA31700.010.4298151
TAAGGCT2300.010.3274864
GGACCTG17150.010.3041946
GTCCTAC26750.010.2643331
TTAGGAC17300.010.1054153
TCCTACA30250.010.0509482
GTCCTAA16250.09.9976841
TTTAGGC1252.5669287E-69.8813383
TATGTCG1554.0145096E-89.81458216
Produced by FastQC (version 0.11.5)