##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512326_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537961 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.010518680153083 31.0 30.0 34.0 16.0 34.0 2 29.91994203220617 31.0 30.0 34.0 23.0 34.0 3 30.59615494485534 33.0 31.0 34.0 26.0 34.0 4 34.26102725770806 37.0 35.0 37.0 31.0 37.0 5 34.427817054714396 37.0 35.0 37.0 32.0 37.0 6 34.58386988610148 37.0 35.0 37.0 32.0 37.0 7 34.523578573508416 37.0 35.0 37.0 32.0 37.0 8 34.525217290573025 37.0 35.0 37.0 32.0 37.0 9 36.10731055362947 39.0 37.0 39.0 32.0 39.0 10 36.04021811209865 39.0 37.0 39.0 32.0 39.0 11 36.06556917147269 39.0 37.0 39.0 32.0 39.0 12 36.00604698023334 39.0 37.0 39.0 32.0 39.0 13 35.97848233286485 39.0 37.0 39.0 32.0 39.0 14 37.21922243880028 40.0 38.0 41.0 32.0 41.0 15 37.146672860615276 40.0 38.0 41.0 31.0 41.0 16 37.07487900720302 40.0 38.0 41.0 31.0 41.0 17 37.02621632089697 40.0 38.0 41.0 31.0 41.0 18 36.80923583932141 40.0 38.0 41.0 30.0 41.0 19 36.91828519035556 40.0 38.0 41.0 31.0 41.0 20 36.85095161036754 40.0 38.0 41.0 31.0 41.0 21 36.7957691233238 40.0 38.0 41.0 31.0 41.0 22 36.723959509228074 40.0 38.0 41.0 30.0 41.0 23 36.595528457687095 40.0 37.0 41.0 30.0 41.0 24 36.466635618120215 40.0 37.0 41.0 30.0 41.0 25 36.35356886886757 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0751754208800932 1101 2 0.2527553160184759 1101 3 -0.2894339228927443 1101 4 -0.36326658163872594 1101 5 -0.5282743037778488 1101 6 -0.5376762045259298 1101 7 -0.6260896650738346 1101 8 -0.7279284580386829 1101 9 -0.7517453907792131 1101 10 -0.8204795547173376 1101 11 -0.8972444500228107 1101 12 -1.0325864242505034 1101 13 -1.076701032938999 1101 14 -1.2897094686634034 1101 15 -1.2733223993783938 1101 16 -1.3558636373801676 1101 17 -1.5182962464237164 1101 18 -1.376127886645861 1101 19 -1.4012787113857925 1101 20 -1.6768287287426134 1101 21 -1.473827735276025 1101 22 -1.6418197928565732 1101 23 -1.6844912292365564 1101 24 -1.7884105837174076 1101 25 -1.928012642449545 1102 1 1.2501338913500923 1102 2 0.39215155781186084 1102 3 -0.10905917284698319 1102 4 -0.18061090202221664 1102 5 -0.25298670530710154 1102 6 -0.38671040551849956 1102 7 -0.4490652515813096 1102 8 -0.5399096842736029 1102 9 -0.5117610010474536 1102 10 -0.5091358628888969 1102 11 -0.6560024321538904 1102 12 -0.8499575100275436 1102 13 -0.6594371814161306 1102 14 -0.9179391882656844 1102 15 -0.8513452483904871 1102 16 -0.9345708170674811 1102 17 -1.1346989429939498 1102 18 -1.1001469781420639 1102 19 -1.1914962190614133 1102 20 -1.332198730089516 1102 21 -1.4388066855890997 1102 22 -1.5709901001923612 1102 23 -1.5093405659728347 1102 24 -1.4102357763418993 1102 25 -1.5494731350018611 1103 1 1.0585806054141145 1103 2 0.22188918818466163 1103 3 -0.12427625033083345 1103 4 -0.15056651211279615 1103 5 -0.3672654595024838 1103 6 -0.560076459869066 1103 7 -0.7056732128935224 1103 8 -0.6518299989727936 1103 9 -0.8499760214379464 1103 10 -0.8400211348222086 1103 11 -0.8265444942997604 1103 12 -1.0222029898355487 1103 13 -0.9557668937839523 1103 14 -1.1207830296168737 1103 15 -1.0416714961800153 1103 16 -1.117753176589659 1103 17 -1.2742474651347706 1103 18 -1.1279826993878714 1103 19 -1.2576462064301381 1103 20 -1.2507252016330597 1103 21 -1.241392488035956 1103 22 -1.519719754817217 1103 23 -1.499351031886441 1103 24 -1.5383692347850086 1103 25 -1.660853913810186 1104 1 1.1793109035493927 1104 2 0.42025464662628664 1104 3 -0.025086781960403925 1104 4 0.045862459522744814 1104 5 -0.24434164652925006 1104 6 -0.2525624804435367 1104 7 -0.4629414249360053 1104 8 -0.43113852147379816 1104 9 -0.45220727551375717 1104 10 -0.6239296754718922 1104 11 -0.5771175962848787 1104 12 -0.5510950563989141 1104 13 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1.2501515376517531 1201 16 1.2852638936763228 1201 17 1.2543644178875937 1201 18 1.3273461348055946 1201 19 1.2651520821323388 1201 20 1.2426681202318832 1201 21 1.2364091087176092 1201 22 1.2119391927369279 1201 23 1.289484043760865 1201 24 1.330691680040104 1201 25 1.2932725031983239 1202 1 2.5239615154720134 1202 2 1.6587070745773325 1202 3 1.2307363526625714 1202 4 0.9740109001070323 1202 5 0.8314578007664508 1202 6 0.7711248468363721 1202 7 0.6882626808673891 1202 8 0.678217972801761 1202 9 0.8048002254732154 1202 10 0.9046925116579914 1202 11 0.9349234617490723 1202 12 0.9064651488912574 1202 13 0.9276561616618508 1202 14 1.098905568384545 1202 15 1.0812543542105502 1202 16 1.1572303007394495 1202 17 1.0863705430954553 1202 18 1.3138654136580783 1202 19 1.2115695932083597 1202 20 1.0668457831620088 1202 21 1.1182257751848823 1202 22 1.1569124946466687 1202 23 1.2054803271633858 1202 24 1.1816092250121244 1202 25 1.3719271906173205 1203 1 2.474884182887891 1203 2 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2314 10 -0.28068128633305633 2314 11 -1.297036493728804 2314 12 -0.7754451642263618 2314 13 -0.5613804469957628 2314 14 -0.5795491610379173 2314 15 -0.7586951917013067 2314 16 -0.7898270776171827 2314 17 -1.117364917892374 2314 18 -1.876056761761859 2314 19 -1.7291885876513717 2314 20 -2.0224726476936965 2314 21 -1.3892621782195036 2314 22 -1.0712218048544457 2314 23 -0.9365123554000903 2314 24 -0.8731281121341183 2314 25 -0.6579226505284765 2315 1 -2.564218336011539 2315 2 -1.0551563415336567 2315 3 -0.4579082861161581 2315 4 -0.3721001859879891 2315 5 -0.19927463889910513 2315 6 -0.22456714995831106 2315 7 -0.16666797278904966 2315 8 -0.24683522129318192 2315 9 -0.237219589828932 2315 10 -0.26479591165239924 2315 11 -0.3745268370419339 2315 12 -0.3913973484893276 2315 13 -0.4619547613631809 2315 14 -0.39601680552825513 2315 15 -1.0511361401876513 2315 16 -0.9259302761385371 2315 17 -0.8231234582209694 2315 18 -0.5429986927806141 2315 19 -0.4545296154803964 2315 20 -0.5728172373224467 2315 21 -0.5591893158463321 2315 22 -0.4072425450655004 2315 23 -0.5188604087215225 2315 24 -0.4724656224275492 2315 25 -0.1974168840427879 2316 1 1.1758297610345316 2316 2 0.6033199579162734 2316 3 -0.020287083789074956 2316 4 -0.17050124402393152 2316 5 -0.3544507677060693 2316 6 -0.568490749460004 2316 7 -0.4351817443454138 2316 8 -0.5375622990385125 2316 9 -0.5895762707860186 2316 10 -0.5961330442065673 2316 11 -0.647382221459182 2316 12 -0.4865636201261907 2316 13 -0.6300170349021244 2316 14 -0.6613059450567462 2316 15 -0.6629419588900234 2316 16 -0.6234266044665091 2316 17 -0.591896042409374 2316 18 -0.509578428123028 2316 19 -0.63070547928303 2316 20 -0.5629823573050103 2316 21 -0.5769272686755684 2316 22 -0.7846607095019138 2316 23 -0.8477208653710235 2316 24 -0.8949642416807322 2316 25 -0.8549966268152289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68724.0 3 3.0 4 561.0 5 2717.0 6 3260.0 7 3120.0 8 2879.0 9 2675.0 10 2638.0 11 2727.0 12 2749.0 13 2825.0 14 2828.0 15 2830.0 16 2893.0 17 3076.0 18 3569.0 19 4424.0 20 5212.0 21 6434.0 22 7841.0 23 9509.0 24 11097.0 25 13190.0 26 14913.0 27 16578.0 28 19003.0 29 22847.0 30 29057.0 31 38165.0 32 50501.0 33 68519.0 34 95925.0 35 138972.0 36 240856.0 37 409240.0 38 1225604.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62186436898317 19.449215469508406 12.434503842388528 24.494416319119896 2 14.550076684176977 20.060470047725577 41.72480714354306 23.664646124554388 3 18.45482552288587 25.0507331904556 29.660002640418483 26.83443864624005 4 12.411389843584498 16.89070834374101 38.24421465000174 32.45368716267276 5 13.350151426060455 37.765469800142895 35.417061828619836 13.46731694517681 6 31.555182849996722 36.84472266572077 18.38260446398735 13.21749002029516 7 27.902585735118734 31.576241858287545 22.552385335901988 17.968787070691732 8 25.231464554093513 36.766370116397326 20.70704056217931 17.29512476732985 9 26.846772890518427 15.158369878810312 20.448611614252005 37.546245616419256 10 15.731010273512727 28.516304442271327 34.02747607770776 21.72520920650819 11 34.01966403423569 22.199281331728567 25.12380378310075 18.657250850934993 12 23.93650638159371 24.277587476339598 31.611759259551853 20.174146882514844 13 29.131919258005535 21.946257868045972 25.731177137113786 23.190645736834703 14 21.271550889768005 22.11051564115996 27.5243409206371 29.093592548434938 15 23.00534138990011 30.985992344363666 23.315093765927 22.69357249980922 16 22.27280747353571 27.51767780465284 28.004870742550874 22.204643979260574 17 21.866108167418407 28.357870554108437 27.79805234862097 21.97796892985218 18 21.682803085979245 26.85201950808478 30.088810465220405 21.37636694071557 19 23.844632061721683 26.421261374154952 27.497697747145956 22.236408816977406 20 24.07967733631909 26.76484336630192 28.085060854761863 21.070418442617125 21 23.262076526549468 26.202401862491616 27.12421203568486 23.411309575274057 22 23.860893433472718 27.601165022032585 27.08332922289905 21.454612321595643 23 22.530170932405262 27.445455299615627 27.914561449942916 22.10981231803619 24 23.551932404399977 26.30919419420607 28.299805213446678 21.839068187947277 25 22.940440451329895 26.979157065509135 28.068833228437594 22.011569254723373 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1069.0 1 1069.0 2 1635.5 3 2202.0 4 2202.0 5 2202.0 6 3496.5 7 4791.0 8 4791.0 9 4791.0 10 5679.0 11 6567.0 12 6567.0 13 6567.0 14 9115.5 15 11664.0 16 11664.0 17 11664.0 18 20691.0 19 29718.0 20 29718.0 21 29718.0 22 46602.5 23 63487.0 24 63487.0 25 63487.0 26 91888.0 27 120289.0 28 120289.0 29 120289.0 30 155543.5 31 190798.0 32 190798.0 33 190798.0 34 213789.5 35 236781.0 36 236781.0 37 236781.0 38 254605.5 39 272430.0 40 272430.0 41 272430.0 42 288162.5 43 303895.0 44 303895.0 45 303895.0 46 313658.5 47 323422.0 48 323422.0 49 323422.0 50 316068.5 51 308715.0 52 308715.0 53 308715.0 54 277941.0 55 247167.0 56 247167.0 57 247167.0 58 213892.5 59 180618.0 60 180618.0 61 180618.0 62 149790.5 63 118963.0 64 118963.0 65 118963.0 66 92232.0 67 65501.0 68 65501.0 69 65501.0 70 48017.5 71 30534.0 72 30534.0 73 30534.0 74 21559.0 75 12584.0 76 12584.0 77 12584.0 78 8533.5 79 4483.0 80 4483.0 81 4483.0 82 2973.5 83 1464.0 84 1464.0 85 1464.0 86 984.0 87 504.0 88 504.0 89 504.0 90 342.0 91 180.0 92 180.0 93 180.0 94 129.5 95 79.0 96 79.0 97 79.0 98 67.5 99 56.0 100 56.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34693204505506586 2 0.2936215331914084 3 0.3175383703689694 4 0.30642708851712064 5 0.28972076403065294 6 0.24913700407531875 7 0.3549699936287437 8 0.31883862675588787 9 0.261627345731475 10 0.23692247438002398 11 0.2495704228709582 12 0.24842777331881774 13 0.26938948234429133 14 0.2611151235184465 15 0.34846871169415133 16 0.40248845431430974 17 0.42207110353547594 18 0.11867794658783172 19 0.13739375821771885 20 0.1987816203637487 21 0.19740256055944122 22 0.14815042469131715 23 0.2581205936576646 24 0.3561520448895787 25 0.3961447792144955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2537961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.3362000868899 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2771450107613 49.57776918632074 2 13.438999804285078 17.023503611437484 3 4.134162628653716 7.8552645432046315 4 1.648675051870427 4.176832518541158 5 0.8201530418843966 2.5972688581330767 6 0.44784715157056937 1.7018962080130373 7 0.2791555952720042 1.2376458246265758 8 0.18105863077877118 0.9174052533170061 9 0.13125499106024807 0.7481873138575336 >10 0.5859545951363366 6.428903116533354 >50 0.029824398595535276 1.284129553431415 >100 0.021774418325752116 2.7319373778585456 >500 0.0021845916125823243 1.004243963098974 >1k 0.0016852563868362904 2.2142545611473015 >5k 1.2483380643231782E-4 0.5007581104792428 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7618 0.30016221683469524 No Hit GTCCTACAGTGGACATTTCTAAATT 5049 0.19893922719852666 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4798 0.18904939831620737 No Hit TATCAACGCAGAGTACTTTTTTTTT 4525 0.1782927318426091 No Hit CTGTAGGACGTGGAATATGGCAAGA 4465 0.17592862932093914 No Hit GGTATCAACGCAGAGTACTTTTTTT 4406 0.17360392850796366 No Hit CTTTAGGACGTGAAATATGGCGAGG 4178 0.16462033892561784 No Hit GTCCTACAGTGTGCATTTCTCATTT 2987 0.11769290387046924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.940170869449925E-5 0.0 2 0.0 0.0 0.0 3.940170869449925E-5 0.0 3 0.0 0.0 0.0 3.940170869449925E-5 0.0 4 0.0 3.940170869449925E-5 0.0 3.940170869449925E-5 0.0 5 0.0 3.940170869449925E-5 0.0 3.940170869449925E-5 0.0 6 0.0 3.940170869449925E-5 0.0 3.940170869449925E-5 0.0 7 0.0 3.940170869449925E-5 0.0 3.940170869449925E-5 0.0 8 0.0 3.940170869449925E-5 0.0 3.940170869449925E-5 0.0 9 0.0 3.940170869449925E-5 0.0 1.1820512608349774E-4 0.0 10 0.0 3.940170869449925E-5 0.0 1.1820512608349774E-4 0.0 11 0.0 1.1820512608349774E-4 0.0 1.9700854347249624E-4 0.0 12 0.0 1.1820512608349774E-4 0.0 1.9700854347249624E-4 0.0 13 0.0 2.3641025216699548E-4 0.0 1.9700854347249624E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 13.416562 1 CCGGTTT 120 1.0080839E-8 11.870497 12 GGACGGG 105 2.7188798E-7 11.763033 6 TAACGTG 65 8.017352E-4 11.693892 5 TAGGACC 1045 0.0 11.547018 4 AACCGTG 60 0.0058723264 11.084616 7 TCATTCG 60 0.0059357947 11.068617 9 GTATAAG 275 0.0 11.055387 1 CGGTTTC 130 3.25781E-8 10.964109 13 AGGACCT 1760 0.0 10.95887 5 GGCGAGG 1155 0.0 10.531859 19 GTATCAA 3170 0.0 10.429815 1 TAAGGCT 230 0.0 10.327486 4 GGACCTG 1715 0.0 10.304194 6 GTCCTAC 2675 0.0 10.264333 1 TTAGGAC 1730 0.0 10.105415 3 TCCTACA 3025 0.0 10.050948 2 GTCCTAA 1625 0.0 9.997684 1 TTTAGGC 125 2.5669287E-6 9.881338 3 TATGTCG 155 4.0145096E-8 9.814582 16 >>END_MODULE