FastQCFastQC Report
Thu 26 May 2016
SRR1512326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537961
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86930.34251905368128194No Hit
TATCAACGCAGAGTACTTTTTTTTT57090.2249443549368962No Hit
GGTATCAACGCAGAGTACTTTTTTT53210.2096564919634305No Hit
GTCCTACAGTGGACATTTCTAAATT50210.19783597935508068No Hit
GTCCTAAAGTGTGTATTTCTCATTT49300.19425042386388128No Hit
CTGTAGGACGTGGAATATGGCAAGA47110.18562144965978594No Hit
CTTTAGGACGTGAAATATGGCGAGG45870.18073563778166804No Hit
GTCCTACAGTGTGCATTTCTCATTT30660.12080563885733468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.014.5121791
TACGGCG500.001506042313.29291116
ACGGCGA655.482495E-513.14683417
TAGGACC9450.012.8697424
AATACCG658.013294E-411.6946025
GGCGAGG12950.011.29105419
CCGTCCG600.00586107211.0874769
AGGACGT26800.010.9555065
TGTAGGA27950.010.8096412
GGACGTG26650.010.7701386
GACGTGA14350.010.663157
TTAGGAC18000.010.5567933
TACCGTA1002.388579E-510.4545237
GTATCAA34100.010.2340141
TCCAACG1409.77725E-810.17274618
GTAGGAC28750.010.1455363
ATACCGT750.002639065510.137726
GACGTGG12950.010.0545447
GTATTAA3800.010.0094611
CTGTAGG28600.010.0077111
Produced by FastQC (version 0.11.5)