FastQCFastQC Report
Thu 26 May 2016
SRR1512325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760327
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66440.37742987524476984No Hit
GTCCTACAGTGGACATTTCTAAATT46000.2613150852086005No Hit
GTCCTAAAGTGTGTATTTCTCATTT43280.24586341060496147No Hit
CTGTAGGACGTGGAATATGGCAAGA41950.23830799618479978No Hit
CTTTAGGACGTGAAATATGGCGAGG38650.21956147920244362No Hit
TATCAACGCAGAGTACTTTTTTTTT38300.21757321224976953No Hit
GGTATCAACGCAGAGTACTTTTTTT37320.21200606478228193No Hit
GTCCTACAGTGTGCATTTCTCATTT24310.1380993417700234No Hit
CTGAAGGACCTGGAATATGGCGAGA18870.10719599256274544No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17980.10214011374023121No Hit
CTGTAGGACCTGGAATATGGCGAGA17860.10145842221360007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17630.10015184678755708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.014.3808841
TAGGACC7800.013.6422364
CGGAGTG500.00149964613.300045
CGTGCGC905.468173E-712.65478110
TTAAGAC1650.012.0923263
TAAGACG550.003066674812.0919824
GGCGAAA752.0619736E-411.40720119
GTATCAA25650.010.81671
TTATACC1158.806983E-710.7400834
TGTAGGA26500.010.6125642
TTATACT2600.010.5970944
CGCCCTA909.422205E-510.56584516
GGCGAGG11850.010.50874219
AGGACCT14950.010.4214365
GAATAAC1106.032902E-610.3654441
CTATTAG1106.032902E-610.3654441
GTCCTAC22400.010.35001851
GTACTAA1201.5202368E-610.2934611
ATTTCTA17300.010.27820715
GTAGGAC25950.010.2516833
Produced by FastQC (version 0.11.5)