FastQCFastQC Report
Thu 26 May 2016
SRR1512325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760327
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78530.4461102965528564No Hit
TATCAACGCAGAGTACTTTTTTTTT48590.27602826065838904No Hit
GTCCTACAGTGGACATTTCTAAATT46820.26597331064057983No Hit
GGTATCAACGCAGAGTACTTTTTTT46640.26495077335063316No Hit
CTGTAGGACGTGGAATATGGCAAGA44900.25506624621448176No Hit
GTCCTAAAGTGTGTATTTCTCATTT44490.25273713349849203No Hit
CTTTAGGACGTGAAATATGGCGAGG40210.22842346904864835No Hit
GTCCTACAGTGTGCATTTCTCATTT24940.14167822228483687No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23150.13150965701258913No Hit
CTGAAGGACCTGGAATATGGCGAGA21350.12128428411312217No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20820.11827347987050134No Hit
CTGTAGGACCTGGAATATGGCGAGA19540.11100210358643593No Hit
ATTTAGAAATGTCCACTGTAGGACG18150.10310584340295867No Hit
TTTCTAAATTTTCCACCTTTTTCAG18120.10293542052130088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.7942141
TCCAACG1450.013.09414318
TAGGACC8550.013.0051754
TGTACCG657.997593E-411.6969635
GGCGAGG10850.011.287230519
GTACCGT855.295872E-511.1821926
CTAGGAC2300.011.1566133
TAGGACT2750.011.0589474
GTATCAA27650.011.0443171
AGGACCT15800.011.0075455
ACGGTAC951.35437695E-511.0043793
TAGAAAT7750.010.91402154
TTTAGAA8200.010.894582
TAGAACG700.001486626210.8614654
CGCCCTA1408.443749E-910.84943316
ATTTAGA7300.010.6861181
CCAACGT803.7950487E-410.68021319
GGACCTG15750.010.3799146
CATTTCG1106.0866605E-610.35774814
GTCGAGG1106.0949187E-610.3565719
Produced by FastQC (version 0.11.5)