FastQCFastQC Report
Thu 26 May 2016
SRR1512324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566284
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77220.4930140383225519No Hit
TATCAACGCAGAGTACTTTTTTTTT44750.2857080835914815No Hit
GGTATCAACGCAGAGTACTTTTTTT44060.28130275224671897No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22660.14467363517727308No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20890.13337300259723014No Hit
GTACTGGTTCACTATCGGTCAGTCA17900.1142832334365926No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15810.10093954863868877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.015.0379081
CCTCGCG500.001517603313.2788959
TAGGACC3350.012.4786024
TCCTAGA2400.011.4808152
GTCCTAG2200.011.2296061
GTCTTAC855.311396E-511.1787931
ATTATAC1553.110472E-1011.0338423
GTATCAA26750.010.7274631
GTCCTAA5200.010.5983551
CAATACA1553.6543497E-910.4201824
TCGTCAA750.002672722310.12179410
AGGACCT7400.010.0143095
GTATAGA2002.5465852E-119.9770731
TTAGACA1554.0556188E-89.8072314
TTATACT1554.0556188E-89.8072314
GTAGGAC10100.09.7835833
GTCCTAC8950.09.7673931
ATTTAGA2750.09.6747371
TGTAGGA10950.09.5447492
TGTCGAA900.00110456919.50838314
Produced by FastQC (version 0.11.5)