FastQCFastQC Report
Thu 26 May 2016
SRR1512324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566284
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92640.5914636170707228No Hit
TATCAACGCAGAGTACTTTTTTTTT56500.3607264072160605No Hit
GGTATCAACGCAGAGTACTTTTTTT55850.35657645739853056No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27450.17525557306337802No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23300.14875973961299482No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19520.12462618528951326No Hit
GTACTGGTTCACTATCGGTCAGTCA18160.11594321336360455No Hit
GTCCTACAGTGGACATTTCTAAATT15840.10113108478411323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.013.7463871
CTTAGAC1103.7956852E-812.0949563
TTATACT2000.011.4038164
CGCCCTA2250.011.39252916
GTGGTAT4100.011.36726951
CTTATAC1104.946214E-711.231033
CCTATAC855.304564E-511.1802133
TCAGTAC951.3550631E-511.0036833
ATTGGAC700.001487253910.8607773
TAGGACC3250.010.52664
GTCCTAA5350.010.4891961
GTATCAA29850.010.2920551
GTCCTAC8950.010.202161
CTATACT1409.636642E-810.1819784
CCATTAG750.002621402310.1454811
TAATACT1701.54796E-910.0621914
TCAATAC1151.0220787E-59.9163633
GTTATAC1151.0220787E-59.9163633
GACGTGA4700.09.9091547
TGGCGAG8350.09.89139918
Produced by FastQC (version 0.11.5)