FastQCFastQC Report
Thu 26 May 2016
SRR1512323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641668
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56130.341908351749562No Hit
GTCCTACAGTGGACATTTCTAAATT54660.3329540443012838No Hit
GTCCTAAAGTGTGTATTTCTCATTT48020.292507376643755No Hit
CTGTAGGACGTGGAATATGGCAAGA43410.26442618117670563No Hit
CTTTAGGACGTGAAATATGGCGAGG41450.25248710457900136No Hit
GGTATCAACGCAGAGTACTTTTTTT35040.21344145101201947No Hit
TATCAACGCAGAGTACTTTTTTTTT33170.20205059731931183No Hit
GTCCTACAGTGTGCATTTCTCATTT31900.1943145629932483No Hit
CTGTAGGACCTGGAATATGGCGAGA23480.14302526454800848No Hit
CTGAAGGACCTGGAATATGGCGAGA20200.12304558534368704No Hit
GTCCTTCAGTGTGCATTTCTCATTT18690.11384762327096587No Hit
ATTTAGAAATGTCCACTGTAGGACG18280.11135016337042569No Hit
TTTCTAAATTTTCCACCTTTTTCAG17100.10416235194935883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC250.0060285619.001993
ACGGCGA653.3409997E-614.62855717
TACGGCG551.9453256E-413.83063516
GGTATCA10000.013.6772471
TAGGACC8900.013.4504354
ACGGTCC500.001515441113.2814728
GGCGAAA751.471533E-512.67264819
CGGTAGG604.099182E-412.6641171
CCGGTTT751.4822239E-512.66372912
TTGCACC1052.7173883E-711.7627764
GCGACTA907.3866468E-611.62157616
GTTTAGG2300.011.5628881
TGTCGAG1001.9257404E-611.40189218
GGCGAGG11700.011.2104219
GCCGGTT855.3539294E-511.17011811
CTAGCAC1201.2733108E-711.0844943
CGACTAA951.3466868E-511.00991317
ATACGGC951.3480592E-511.00890315
TAAGCGA951.3581635E-511.00148913
GTCCAGT1303.2752723E-810.9593311
Produced by FastQC (version 0.11.5)