##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1641668 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.19292938645329 31.0 30.0 34.0 16.0 34.0 2 30.107208034754894 33.0 30.0 34.0 24.0 34.0 3 30.81577334759525 33.0 31.0 34.0 27.0 34.0 4 34.48202194353548 37.0 35.0 37.0 32.0 37.0 5 34.661625858577985 37.0 35.0 37.0 32.0 37.0 6 34.817227965703175 37.0 35.0 37.0 32.0 37.0 7 34.756712684903405 37.0 35.0 37.0 32.0 37.0 8 34.75649217746828 37.0 35.0 37.0 32.0 37.0 9 36.375568019843236 39.0 37.0 39.0 32.0 39.0 10 36.30703954758209 39.0 37.0 39.0 32.0 39.0 11 36.33402307896603 39.0 37.0 39.0 32.0 39.0 12 36.27779002819084 39.0 37.0 39.0 32.0 39.0 13 36.2613847623271 39.0 37.0 39.0 32.0 39.0 14 37.53182251222537 40.0 38.0 41.0 32.0 41.0 15 37.47119697770804 40.0 38.0 41.0 32.0 41.0 16 37.41058606246817 40.0 38.0 41.0 32.0 41.0 17 37.37554060869798 40.0 38.0 41.0 32.0 41.0 18 37.16051966658301 40.0 38.0 41.0 31.0 41.0 19 37.267246483454635 40.0 38.0 41.0 32.0 41.0 20 37.20480389457552 40.0 38.0 41.0 32.0 41.0 21 37.16053976808953 40.0 38.0 41.0 32.0 41.0 22 37.092455965518 40.0 38.0 41.0 32.0 41.0 23 36.98676163511745 40.0 38.0 41.0 31.0 41.0 24 36.87805207873943 40.0 38.0 41.0 31.0 41.0 25 36.76979815650911 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2990402938941052 1101 2 0.4341167043593863 1101 3 -0.07862456784823735 1101 4 -0.09165856060936761 1101 5 -0.2742179437102905 1101 6 -0.2964492672863628 1101 7 -0.30501103315855715 1101 8 -0.4644491028028668 1101 9 -0.4439585177564922 1101 10 -0.5511681829037443 1101 11 -0.6870220300213745 1101 12 -0.7521994733921744 1101 13 -0.8228411309541173 1101 14 -1.2095993377686938 1101 15 -1.1704368261882507 1101 16 -1.357292673697195 1101 17 -1.4743251318784019 1101 18 -1.1561018512812495 1101 19 -1.4050382147883909 1101 20 -1.5178632100386409 1101 21 -1.4083201643527303 1101 22 -1.646271378182469 1101 23 -1.6215780192283589 1101 24 -1.743088420032322 1101 25 -1.9449876882121586 1102 1 1.0636981484426187 1102 2 0.23964023694641057 1102 3 -0.2590021887210021 1102 4 -0.1648133368841016 1102 5 -0.3850588563151689 1102 6 -0.4747341683286095 1102 7 -0.40844989309868396 1102 8 -0.5352245348416886 1102 9 -0.7218006428639754 1102 10 -0.7924932737510275 1102 11 -0.5744952776567231 1102 12 -0.8467923085053854 1102 13 -0.6430444444045804 1102 14 -0.9297888172967532 1102 15 -0.8867351759731008 1102 16 -0.9541821207652603 1102 17 -1.101921817885433 1102 18 -1.033430674575044 1102 19 -1.1658438192449054 1102 20 -1.2776272015140506 1102 21 -1.3278953453917524 1102 22 -1.2224723299201656 1102 23 -1.3495434289972792 1102 24 -1.2178369907530495 1102 25 -1.44763602538373 1103 1 1.1663695590867427 1103 2 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1108 21 -1.1005150979800788 1108 22 -1.1568906651948723 1108 23 -1.0940633774586033 1108 24 -1.1564330824033888 1108 25 -1.252085954127601 1109 1 0.8974417159419801 1109 2 0.3611840371001982 1109 3 -0.10314304323311774 1109 4 -0.08568677681785175 1109 5 -0.07015162233973626 1109 6 9.25052081882427E-4 1109 7 -0.2073796827238752 1109 8 -0.20554689924161806 1109 9 -0.21747287614933697 1109 10 -0.22946009092013497 1109 11 -0.2535867423938072 1109 12 -0.4435844376877398 1109 13 -0.3531097059436519 1109 14 -0.37155383632315164 1109 15 -0.7153402352211131 1109 16 -0.5800107057137041 1109 17 -0.5308817737205302 1109 18 -0.6070512112575486 1109 19 -0.6339086470214781 1109 20 -0.6496175798732438 1109 21 -0.5979645502262372 1109 22 -0.7701779234847024 1109 23 -0.7132241009348164 1109 24 -0.7446163042253175 1109 25 -0.9299952990287181 1110 1 0.6882254019686691 1110 2 0.22357292829880393 1110 3 -0.12049805092446775 1110 4 -0.1169028413420321 1110 5 -0.025398784855845236 1110 6 -0.1606148805668468 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2314 8 -0.3856659450891513 2314 9 -0.442320720675454 2314 10 -0.48796695837746284 2314 11 -1.64329284795771 2314 12 -1.1027740330806637 2314 13 -0.7972891080358764 2314 14 -0.854522542145979 2314 15 -0.8822190019061367 2314 16 -1.0362970704999412 2314 17 -1.2434503366883405 2314 18 -2.1937561710051696 2314 19 -1.9683438488785967 2314 20 -2.2449372923775215 2314 21 -1.5783954961943039 2314 22 -1.2283806288015882 2314 23 -0.9199385843502412 2314 24 -0.8733512932879819 2314 25 -0.9599261036298401 2315 1 -2.6673638982358554 2315 2 -1.2926917288740185 2315 3 -0.6432106778030295 2315 4 -0.6023289492605812 2315 5 -0.464281399349062 2315 6 -0.5814851287007414 2315 7 -0.5098735167903357 2315 8 -0.5034433624112893 2315 9 -0.5086638548164757 2315 10 -0.4189304562993712 2315 11 -0.684288260357647 2315 12 -0.6602578847624159 2315 13 -0.7048908343730531 2315 14 -0.7579778491381219 2315 15 -1.3030663406395107 2315 16 -1.0713936546826588 2315 17 -0.9730626072021806 2315 18 -0.655689366308529 2315 19 -0.8497486124413953 2315 20 -0.8125398509709072 2315 21 -0.8074158457079434 2315 22 -0.8429351764023991 2315 23 -0.9259788111995064 2315 24 -0.7706462058246473 2315 25 -0.6682487506801706 2316 1 1.4615344691336247 2316 2 0.6271693226425619 2316 3 0.14332312964315008 2316 4 -0.0053078733303024705 2316 5 -0.1704868539125428 2316 6 -0.30941650695584144 2316 7 -0.21948648040628171 2316 8 -0.16998505757712223 2316 9 -0.28088400008079617 2316 10 -0.2989061275698006 2316 11 -0.4338467887952149 2316 12 -0.3230114919426512 2316 13 -0.3350863563574151 2316 14 -0.3883089634307524 2316 15 -0.32459967368961884 2316 16 -0.21769386100954335 2316 17 -0.27492117769814683 2316 18 -0.1938821438762517 2316 19 -0.3143639763598216 2316 20 -0.24690644725702526 2316 21 -0.2992516310316873 2316 22 -0.4703823874491704 2316 23 -0.4400503189718137 2316 24 -0.44037839154433556 2316 25 -0.4004532337937903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39273.0 3 3.0 4 324.0 5 1572.0 6 1949.0 7 1782.0 8 1606.0 9 1613.0 10 1551.0 11 1575.0 12 1592.0 13 1651.0 14 1582.0 15 1670.0 16 1702.0 17 1804.0 18 2130.0 19 2437.0 20 3065.0 21 3711.0 22 4581.0 23 5378.0 24 6301.0 25 7400.0 26 8553.0 27 9363.0 28 11063.0 29 13473.0 30 17234.0 31 22403.0 32 29778.0 33 41066.0 34 58686.0 35 86218.0 36 153616.0 37 260221.0 38 833742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.612410739282296 19.8768535237888 12.033891259937723 24.476844476991182 2 14.284431247479745 20.221171352811076 42.90119383652871 22.59320356318047 3 18.577241038778183 25.35780110291961 29.898483485538847 26.16647437276336 4 11.998946581798474 16.903879255299323 39.09965739467193 31.997516768230277 5 12.52334518742436 38.335714944646625 36.3499459631841 12.790993904744916 6 30.987841295625675 37.58706817057606 18.60177953111737 12.823311002680898 7 27.325756598672474 31.658101936890155 23.06364469340264 17.952496771034728 8 24.151995213711565 38.19992287671091 21.00937396300649 16.63870794657104 9 26.736954101230275 14.768301270951708 20.72801364704934 37.766730980768685 10 15.125233023978039 28.68638895724327 35.261740958547534 20.926637060231165 11 33.800532400523 21.798436508581126 26.170618550720036 18.23041254017584 12 23.964153219034824 24.190470374498098 32.63021938596357 19.215157020503504 13 29.364212619730836 22.385772415823464 25.986095892168038 22.263919072277663 14 20.255891277050036 22.99692915809583 28.05795511733694 28.689224447517187 15 22.17082601054482 32.88894322610224 23.390051195843203 21.55017956750974 16 21.102513229131244 27.826190132523728 29.734047367996148 21.337249270348888 17 20.997809465726185 29.221947428400846 28.740898516663904 21.039344589209065 18 20.926308420248308 26.867749589741805 31.823107522101495 20.38283446790839 19 23.47977506160189 26.40812169118544 28.13114494132572 21.980958305886944 20 23.614539009689256 27.25313637462191 28.98287230640409 20.149452309284744 21 22.290724430691107 26.628580496707134 27.723252705399815 23.357442367201948 22 23.196018108601585 28.82692449645289 27.73457636279672 20.242481032148806 23 21.438901381578866 28.457580972784935 28.99422377644586 21.109293869190342 24 23.192688592737497 26.495170558748015 29.636813791417044 20.675327057097444 25 22.175621933447204 27.681427705645177 29.18492914860294 20.95802121230468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 794.0 1 794.0 2 1159.5 3 1525.0 4 1525.0 5 1525.0 6 2457.5 7 3390.0 8 3390.0 9 3390.0 10 3951.5 11 4513.0 12 4513.0 13 4513.0 14 6148.5 15 7784.0 16 7784.0 17 7784.0 18 13748.0 19 19712.0 20 19712.0 21 19712.0 22 31940.0 23 44168.0 24 44168.0 25 44168.0 26 66397.0 27 88626.0 28 88626.0 29 88626.0 30 115304.0 31 141982.0 32 141982.0 33 141982.0 34 157983.0 35 173984.0 36 173984.0 37 173984.0 38 181826.0 39 189668.0 40 189668.0 41 189668.0 42 196246.5 43 202825.0 44 202825.0 45 202825.0 46 206736.5 47 210648.0 48 210648.0 49 210648.0 50 199898.5 51 189149.0 52 189149.0 53 189149.0 54 165003.0 55 140857.0 56 140857.0 57 140857.0 58 120209.5 59 99562.0 60 99562.0 61 99562.0 62 81611.0 63 63660.0 64 63660.0 65 63660.0 66 48550.0 67 33440.0 68 33440.0 69 33440.0 70 24499.0 71 15558.0 72 15558.0 73 15558.0 74 10963.0 75 6368.0 76 6368.0 77 6368.0 78 4295.0 79 2222.0 80 2222.0 81 2222.0 82 1510.0 83 798.0 84 798.0 85 798.0 86 533.0 87 268.0 88 268.0 89 268.0 90 180.5 91 93.0 92 93.0 93 93.0 94 70.0 95 47.0 96 47.0 97 47.0 98 37.0 99 27.0 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3490352495145182 2 0.3001824973137078 3 0.32186775888912983 4 0.31011142325975777 5 0.293664736109859 6 0.2532789821084409 7 0.3469032715506424 8 0.3244870461018915 9 0.2674718639822425 10 0.2412789918546259 11 0.25455816888676636 12 0.25236527726677993 13 0.27130942431721883 14 0.263877958271709 15 0.35287280984949454 16 0.4044666765752881 17 0.42073062275685463 18 0.11250752283652968 19 0.1284059870814318 20 0.19199984406104037 21 0.19967496473099314 22 0.14905571650297136 23 0.25985765696840046 24 0.35744133405779976 25 0.395025059878124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1641668.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.39936100566229 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66450000583043 55.71534478245537 2 11.624588860153048 15.669797222557111 3 3.0169860770386876 6.100288012661621 4 1.0708568686203 2.8870027469413064 5 0.5260799733930263 1.7728727022282893 6 0.28459290112544683 1.1508827809561644 7 0.16733043413990975 0.7894575036480982 8 0.10732351751394326 0.5786829201055824 9 0.07591869344990661 0.4605184284217617 >10 0.400597243173482 4.831247580723874 >50 0.030151396619735184 1.437402994362785 >100 0.02625875304506697 3.7567975749955704 >500 0.002543919341875619 1.2441153994366112 >1k 0.0020896480308264013 2.9271646563896683 >5k 1.8170852441968705E-4 0.6784246941161695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5613 0.341908351749562 No Hit GTCCTACAGTGGACATTTCTAAATT 5466 0.3329540443012838 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4802 0.292507376643755 No Hit CTGTAGGACGTGGAATATGGCAAGA 4341 0.26442618117670563 No Hit CTTTAGGACGTGAAATATGGCGAGG 4145 0.25248710457900136 No Hit GGTATCAACGCAGAGTACTTTTTTT 3504 0.21344145101201947 No Hit TATCAACGCAGAGTACTTTTTTTTT 3317 0.20205059731931183 No Hit GTCCTACAGTGTGCATTTCTCATTT 3190 0.1943145629932483 No Hit CTGTAGGACCTGGAATATGGCGAGA 2348 0.14302526454800848 No Hit CTGAAGGACCTGGAATATGGCGAGA 2020 0.12304558534368704 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1869 0.11384762327096587 No Hit ATTTAGAAATGTCCACTGTAGGACG 1828 0.11135016337042569 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1710 0.10416235194935883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.091365611073615E-5 2 0.0 0.0 0.0 0.0 6.091365611073615E-5 3 0.0 0.0 0.0 0.0 6.091365611073615E-5 4 0.0 0.0 0.0 0.0 6.091365611073615E-5 5 0.0 0.0 0.0 0.0 6.091365611073615E-5 6 0.0 0.0 0.0 0.0 6.091365611073615E-5 7 0.0 0.0 0.0 0.0 6.091365611073615E-5 8 0.0 0.0 0.0 0.0 6.091365611073615E-5 9 0.0 0.0 0.0 0.0 1.218273122214723E-4 10 0.0 0.0 0.0 0.0 1.218273122214723E-4 11 0.0 0.0 0.0 0.0 1.218273122214723E-4 12 0.0 6.091365611073615E-5 0.0 0.0 1.218273122214723E-4 13 0.0 6.091365611073615E-5 0.0 0.0 1.218273122214723E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGAC 25 0.00602856 19.00199 3 ACGGCGA 65 3.3409997E-6 14.628557 17 TACGGCG 55 1.9453256E-4 13.830635 16 GGTATCA 1000 0.0 13.677247 1 TAGGACC 890 0.0 13.450435 4 ACGGTCC 50 0.0015154411 13.281472 8 GGCGAAA 75 1.471533E-5 12.672648 19 CGGTAGG 60 4.099182E-4 12.664117 1 CCGGTTT 75 1.4822239E-5 12.663729 12 TTGCACC 105 2.7173883E-7 11.762776 4 GCGACTA 90 7.3866468E-6 11.621576 16 GTTTAGG 230 0.0 11.562888 1 TGTCGAG 100 1.9257404E-6 11.401892 18 GGCGAGG 1170 0.0 11.21042 19 GCCGGTT 85 5.3539294E-5 11.170118 11 CTAGCAC 120 1.2733108E-7 11.084494 3 CGACTAA 95 1.3466868E-5 11.009913 17 ATACGGC 95 1.3480592E-5 11.008903 15 TAAGCGA 95 1.3581635E-5 11.001489 13 GTCCAGT 130 3.2752723E-8 10.959331 1 >>END_MODULE