FastQCFastQC Report
Thu 26 May 2016
SRR1512323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641668
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59810.3643245771983129No Hit
GTCCTACAGTGGACATTTCTAAATT52950.32253780910634794No Hit
CTTTAGGACGTGAAATATGGCGAGG48440.29506575020040593No Hit
CTGTAGGACGTGGAATATGGCAAGA48300.2942129590148556No Hit
GTCCTAAAGTGTGTATTTCTCATTT46540.2834921555393661No Hit
GGTATCAACGCAGAGTACTTTTTTT39300.23939066851519308No Hit
TATCAACGCAGAGTACTTTTTTTTT37800.23025362009858266No Hit
GTCCTACAGTGTGCATTTCTCATTT30980.18871050663106062No Hit
CTGTAGGACCTGGAATATGGCGAGA27940.17019275517339683No Hit
CTGAAGGACCTGGAATATGGCGAGA23910.14564455176077015No Hit
ATTTAGAAATGTCCACTGTAGGACG20690.1260303544931131No Hit
TTTCTAAATTTTCCACCTTTTTCAG18320.11159381799486863No Hit
GTCCTTCAGTGTGCATTTCTCATTT17540.10684255281823121No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17440.10623341625712385No Hit
GAATATGGCAAGAAAACTGAAAATC17120.10428417926158029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG307.717066E-419.0021632
GGTATCA12200.014.573351
CACGTAA551.9608978E-413.8176510
TAGGACC9000.013.0915764
CTAGTTC802.8623575E-511.8767143
GTCCTAA14550.011.2393981
AGGACCT17750.011.1882115
ACGTTGA600.00585760911.08797558
CCAACGT600.005894932411.07851819
TGGTATC2850.011.0012522
GGACCTG17250.010.9089156
GGCGAGG11400.010.74532919
CGACTAA803.7860908E-410.68285719
ATTATAC2100.010.4062643
GTGGTAT3300.010.37208751
CGCGCCT750.002649221310.13263412
TGTAGGA29750.010.1238422
GTATTAC1701.537046E-910.0670261
GTGTAGG2650.010.0459211
CTGTAGG30050.010.0298021
Produced by FastQC (version 0.11.5)