FastQCFastQC Report
Thu 26 May 2016
SRR1512322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193075
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66630.30381997879689476No Hit
CTGTAGGACGTGGAATATGGCAAGA62510.2850335715832792No Hit
GTCCTAAAGTGTGTATTTCTCATTT59040.2692110392941418No Hit
GTATCAACGCAGAGTACTTTTTTTT58020.2645600355664991No Hit
CTTTAGGACGTGAAATATGGCGAGG49090.22384095391174494No Hit
GGTATCAACGCAGAGTACTTTTTTT35790.1631955131493451No Hit
TATCAACGCAGAGTACTTTTTTTTT35410.16146278627041938No Hit
GTCCTACAGTGTGCATTTCTCATTT31220.14235719252647538No Hit
CTGAAGGACCTGGAATATGGCGAGA27750.12653466023733798No Hit
CTGTAGGACCTGGAATATGGCGAGA23820.10861461646318525No Hit
GTCCTTCAGTGTGCATTTCTCATTT23660.107885047251006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9400.013.5440074
GACTGCG852.6994348E-713.4122827
GGTATCA11400.012.8347111
CCATCGC701.0846114E-412.2223219
AGGTCGA701.09885696E-412.20524810
CAGGTCG701.1066324E-412.1960329
GCACCGT907.4611107E-611.611566
GTGTAGG4100.011.586611
ACACCGT855.3228192E-511.1769026
GGTCGAT600.00590639711.07589211
AATCCCG2150.011.05377719
GGCGAGG13900.011.01083519
TGTAGGA32600.010.9873752
TGGTATC3600.010.8206052
GGACGTG33800.010.7933166
GTAGGAC32850.010.7593923
AGGACCT21350.010.7248045
AGGACGT34150.010.7112535
CGTGCGC1252.2641507E-710.63212710
GTCCTAC29400.010.5351451
Produced by FastQC (version 0.11.5)