FastQCFastQC Report
Thu 26 May 2016
SRR1512322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193075
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71460.32584384938955574No Hit
GTCCTACAGTGGACATTTCTAAATT66720.3042303614787456No Hit
CTGTAGGACGTGGAATATGGCAAGA66720.3042303614787456No Hit
GTCCTAAAGTGTGTATTTCTCATTT58620.26729592011217124No Hit
CTTTAGGACGTGAAATATGGCGAGG53680.24477047068613705No Hit
GGTATCAACGCAGAGTACTTTTTTT46100.21020712925914525No Hit
TATCAACGCAGAGTACTTTTTTTTT44850.20450736978899492No Hit
GTCCTACAGTGTGCATTTCTCATTT32970.1503368557846859No Hit
CTGAAGGACCTGGAATATGGCGAGA30190.1376605907230715No Hit
CTGTAGGACCTGGAATATGGCGAGA26290.11987734117620236No Hit
GTCCTTCAGTGTGCATTTCTCATTT23400.10669949728121474No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22440.10232208200813926No Hit
TTTCTAAATTTTCCACCTTTTTCAG22010.10036136475040754No Hit
ATTTAGAAATGTCCACTGTAGGACG21970.10017897244736272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC402.750426E-416.6339687
TCCAACG1600.013.64617118
TAGGACC9800.013.1886794
GGTATCA13900.013.07204151
CCAACGA1551.8189894E-1212.24901819
GCACCGT550.00305728512.0971576
GGACCGT907.4215586E-611.617116
TGGACCG1157.041126E-811.5696085
CGTTTCC1900.011.4925614
CTTATAC2400.011.0875423
CCCGTTT1803.6379788E-1211.08197612
TGTAGGA33050.011.04175952
GGCGAGG14550.011.0262219
ACCTTTT8300.010.98059415
CACCTTT8600.010.92897114
GTCCTAC31350.010.8028241
ATTTAGA8750.010.7634631
GCACCGG1158.7623994E-710.7446796
CTGTAGG33200.010.7166331
ACCGGGC803.7532515E-410.6932668
Produced by FastQC (version 0.11.5)