FastQCFastQC Report
Thu 26 May 2016
SRR1512321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727199
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT104580.38347036648224053No Hit
CTGTAGGACGTGGAATATGGCAAGA93980.34460264909161376No Hit
GTCCTAAAGTGTGTATTTCTCATTT88660.325095455080469No Hit
CTTTAGGACGTGAAATATGGCGAGG75810.277977514658813No Hit
GTATCAACGCAGAGTACTTTTTTTT75240.2758874581576189No Hit
GTCCTACAGTGTGCATTTCTCATTT64040.23481968129205094No Hit
CTGTAGGACCTGGAATATGGCGAGA45110.165407804857658No Hit
GGTATCAACGCAGAGTACTTTTTTT44520.16324441304063253No Hit
TATCAACGCAGAGTACTTTTTTTTT41930.15374748964046994No Hit
CTGAAGGACCTGGAATATGGCGAGA39550.14502058705653675No Hit
GTCCTTCAGTGTGCATTTCTCATTT33800.1239366837550175No Hit
ATTTAGAAATGTCCACTGTAGGACG31500.11550312243440981No Hit
GAATATGGCAAGAAAACTGAAAATC29640.10868293806209227No Hit
TTTCTAAATTTTCCACCTTTTTCAG28060.10288944811141394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17850.014.0529554
GGTATCA13650.013.0169991
TCCAACG2950.012.8846318
CCAACGA2300.012.81208819
GGACCTG30550.011.5700096
TAGAAAT13250.011.4737674
GTACCGT752.0714292E-411.4020576
ACCTTTT12250.011.33221815
AGGACCT32650.011.3205725
CACCTTT12100.011.2369614
GGCGAGG23150.011.20979619
TGTAGGA56450.011.0422562
AATGTCC12850.010.8464798
GAAATGT13250.010.7566576
GTATCAA31850.010.739771
CCACCTT12300.010.73711513
GTCCTAC53550.010.7171541
TTAGGAC30550.010.6995423
GTGTAGG5450.010.6349461
GTCCTAA26250.010.6057221
Produced by FastQC (version 0.11.5)