FastQCFastQC Report
Thu 26 May 2016
SRR1512321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727199
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT104190.3820403278235288No Hit
CTGTAGGACGTGGAATATGGCAAGA100440.3682899561051467No Hit
GTCCTAAAGTGTGTATTTCTCATTT89680.32883555618786897No Hit
GTATCAACGCAGAGTACTTTTTTTT86630.3176519205235848No Hit
CTTTAGGACGTGAAATATGGCGAGG81710.2996114328290675No Hit
GTCCTACAGTGTGCATTTCTCATTT65470.24006315637399395No Hit
GGTATCAACGCAGAGTACTTTTTTT53750.1970886612968104No Hit
TATCAACGCAGAGTACTTTTTTTTT52510.19254187171526535No Hit
CTGTAGGACCTGGAATATGGCGAGA49470.18139490370889694No Hit
CTGAAGGACCTGGAATATGGCGAGA44060.161557700776511No Hit
GTCCTTCAGTGTGCATTTCTCATTT34540.12665009044077824No Hit
ATTTAGAAATGTCCACTGTAGGACG34050.12485337520290965No Hit
GAATATGGCAAGAAAACTGAAAATC32430.11891321462056857No Hit
TTTCTAAATTTTCCACCTTTTTCAG31980.11726317001436272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC18250.013.06893354
GGTATCA16600.012.948051
GACTGCG907.427796E-611.6164957
GGCGAGG22200.011.50498619
ACCTTTT11950.011.20350515
CACCTTT12200.011.12999214
CCACCTT12100.011.06827113
TGTAGGA56850.011.0147912
AATGTCC10400.010.9666218
GACGTGG27300.010.8969937
ACTGCGC700.001486268210.8621778
CTGTAGG56400.010.8595231
AGGACCT33900.010.8201395
AGGACGT53750.010.8197755
CGCCCTA1502.1573214E-910.76020416
GGACGTG52950.010.7519146
TAGAAAT11800.010.7102334
GTCGAGG1605.493348E-1010.68167219
TGGCGAA1605.511538E-1010.68128118
TTAGGAC31000.010.6056123
Produced by FastQC (version 0.11.5)