FastQCFastQC Report
Thu 26 May 2016
SRR1512320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763121
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT130510.4723282114681189No Hit
CTGTAGGACGTGGAATATGGCAAGA117150.42397708967504505No Hit
GTCCTAAAGTGTGTATTTCTCATTT112940.4087406957567186No Hit
CTTTAGGACGTGAAATATGGCGAGG95320.34497222524818855No Hit
GTCCTACAGTGTGCATTTCTCATTT70790.25619580177632467No Hit
GTATCAACGCAGAGTACTTTTTTTT68220.24689472520385464No Hit
CTGAAGGACCTGGAATATGGCGAGA55740.20172840784026472No Hit
CTGTAGGACCTGGAATATGGCGAGA49560.17936239491502542No Hit
GTCCTTCAGTGTGCATTTCTCATTT45000.16285931741679063No Hit
GGTATCAACGCAGAGTACTTTTTTT40690.1472610139042047No Hit
ATTTAGAAATGTCCACTGTAGGACG39130.14161522423375597No Hit
TATCAACGCAGAGTACTTTTTTTTT37390.13531799729364005No Hit
GAATATGGCAAGAAAACTGAAAATC36390.1316989013510447No Hit
TTTCTAAATTTTCCACCTTTTTCAG35130.12713884046337456No Hit
GGAATATGGCGAGAAAACTGAAAAT33680.12189115134661133No Hit
ATTCCAGGTCCTTCAGTGTGCATTT30730.11121481831595503No Hit
ACCTGGAATATGGCGAGAAAACTGA28610.1035423349176529No Hit
TTCCAGGTCCTTCAGTGTGCATTTC27640.10003181185333541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT551.9585634E-413.8202196
TAGGACC18000.013.5661354
GGTATCA14000.012.6909261
CACCTTT13300.012.01160314
ACCTTTT13750.011.89512615
CCACCTT13450.011.65599313
GATATAC10000.011.306461
AATCCCG5300.011.3004919
TCCAACG3650.011.19546718
TGTAGGA63500.011.1780522
ACTGTTC10950.011.1696588
TACTACG600.005867981411.0857742
ATAGGAC4900.011.0534543
GTATTAG2150.011.0479381
AGGACCT38000.011.0268265
TAGGACA15900.010.9357744
AAGACCG700.001489974410.8589415
GGACGGG1408.341885E-910.8587446
CTGTAGG59750.010.7972121
GTGTAGG6250.010.7933941
Produced by FastQC (version 0.11.5)