##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512320_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2763121 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.77211674769219 31.0 30.0 34.0 16.0 34.0 2 29.692788697997663 31.0 30.0 34.0 19.0 34.0 3 30.376719658675825 33.0 31.0 34.0 26.0 34.0 4 34.01179029076179 37.0 35.0 37.0 30.0 37.0 5 34.187151051293085 37.0 35.0 37.0 30.0 37.0 6 34.34098579106742 37.0 35.0 37.0 31.0 37.0 7 34.27994611889961 37.0 35.0 37.0 31.0 37.0 8 34.26994655681022 37.0 35.0 37.0 31.0 37.0 9 35.838513405674234 39.0 37.0 39.0 31.0 39.0 10 35.75727411141242 39.0 37.0 39.0 30.0 39.0 11 35.77736660826652 39.0 37.0 39.0 31.0 39.0 12 35.71397126654967 39.0 37.0 39.0 30.0 39.0 13 35.69714210850701 39.0 37.0 39.0 30.0 39.0 14 36.893236307783845 40.0 38.0 41.0 31.0 41.0 15 36.84588441838052 40.0 38.0 41.0 31.0 41.0 16 36.77725912111703 40.0 38.0 41.0 30.0 41.0 17 36.73773497432794 40.0 38.0 41.0 30.0 41.0 18 36.522021655946304 40.0 37.0 41.0 30.0 41.0 19 36.62074588843558 40.0 38.0 41.0 30.0 41.0 20 36.565691839047226 40.0 38.0 41.0 30.0 41.0 21 36.4998550552075 40.0 37.0 41.0 30.0 41.0 22 36.425601701843675 40.0 37.0 41.0 30.0 41.0 23 36.303039932018905 40.0 37.0 41.0 29.0 41.0 24 36.189457139227706 40.0 37.0 41.0 29.0 41.0 25 36.07437965981222 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8995027776887348 1101 2 0.19090685550173347 1101 3 -0.39473627876057193 1101 4 -0.48599173227339776 1101 5 -0.7222184043895652 1101 6 -0.6896747348691932 1101 7 -0.6949842942644437 1101 8 -0.841162241372011 1101 9 -0.8359841872557894 1101 10 -0.978861474106381 1101 11 -1.0327330443160392 1101 12 -1.2122148728765154 1101 13 -1.133796682814065 1101 14 -1.5014839644435725 1101 15 -1.4179692113166737 1101 16 -1.6017920742157798 1101 17 -1.6036849376051308 1101 18 -1.4633951354326626 1101 19 -1.5675884049834252 1101 20 -1.6054070690400835 1101 21 -1.6751924382711465 1101 22 -1.7726619734787903 1101 23 -1.8756105314307945 1101 24 -2.0862664968299853 1101 25 -2.21224749916761 1102 1 0.8612487987148256 1102 2 0.03492477495424495 1102 3 -0.4870961986182998 1102 4 -0.49430176581609686 1102 5 -0.5940776601469722 1102 6 -0.7295567726441234 1102 7 -0.6987874504293217 1102 8 -0.8440608703988204 1102 9 -0.9278150780506849 1102 10 -0.9747017298412786 1102 11 -0.9858898845492661 1102 12 -1.1771517270670984 1102 13 -1.1161968989280595 1102 14 -1.473317556663467 1102 15 -1.5060878939217517 1102 16 -1.38124638636544 1102 17 -1.4930404226234728 1102 18 -1.232793843251038 1102 19 -1.395346447350775 1102 20 -1.6188309001109715 1102 21 -1.682084673925452 1102 22 -1.6871695262128625 1102 23 -1.9090123008641768 1102 24 -1.8122123332586995 1102 25 -1.8534946570433917 1103 1 1.2038194155952908 1103 2 0.36595734952036096 1103 3 -0.13150305973958254 1103 4 -0.14469017340254453 1103 5 -0.32769797014828583 1103 6 -0.38318514311716 1103 7 -0.38637752144020254 1103 8 -0.35050803984736234 1103 9 -0.5799555582029825 1103 10 -0.54620935495943 1103 11 -0.6544898138638118 1103 12 -0.69229161504758 1103 13 -0.8129983894900477 1103 14 -1.0292506507690504 1103 15 -1.1519022111783386 1103 16 -1.104654397013448 1103 17 -1.1361982801125734 1103 18 -1.0307788390069206 1103 19 -1.0891418878693386 1103 20 -1.1569306268479025 1103 21 -1.29371162017339 1103 22 -1.437000308887221 1103 23 -1.4101584880066724 1103 24 -1.4777529213292269 1103 25 -1.6216438280667873 1104 1 1.1481033313724502 1104 2 0.41389345692815027 1104 3 -0.20691388676108957 1104 4 -0.17675108015702534 1104 5 -0.3878840051793091 1104 6 -0.3631773208725875 1104 7 -0.42249708121067187 1104 8 -0.4831632285600236 1104 9 -0.616010226128104 1104 10 -0.6698903672397023 1104 11 -0.7092986315140735 1104 12 -0.5498021281361503 1104 13 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-0.36030006798146985 2314 15 -0.4985625739998909 2314 16 -0.6301882622671968 2314 17 -0.8648589329020737 2314 18 -1.8358264018977692 2314 19 -1.60530760086737 2314 20 -1.7100889057062147 2314 21 -1.1361029918523613 2314 22 -0.6984714821334705 2314 23 -0.4579486208595327 2314 24 -0.5164006929791398 2314 25 -0.46056094176736906 2315 1 -2.6655183840606362 2315 2 -1.246339254621148 2315 3 -0.5240198462021475 2315 4 -0.41723771971947343 2315 5 -0.2875119086525828 2315 6 -0.1849547446833384 2315 7 -0.25198321061930784 2315 8 -0.21955456180383237 2315 9 -0.39060869399278886 2315 10 -0.3143545326436623 2315 11 -0.5720145671441514 2315 12 -0.5928266212254911 2315 13 -0.47351534400116435 2315 14 -0.5679549616015578 2315 15 -1.0946653204172492 2315 16 -0.8159716730626201 2315 17 -0.5774267847089334 2315 18 -0.4710847222162826 2315 19 -0.564912210418008 2315 20 -0.611979479025976 2315 21 -0.5513990669080044 2315 22 -0.4558769236924576 2315 23 -0.5560877442137198 2315 24 -0.6186574678169166 2315 25 -0.33831039147781894 2316 1 1.149878662482486 2316 2 0.4518565341283711 2316 3 -0.11611228559484132 2316 4 -0.2221301669003637 2316 5 -0.42976844100171974 2316 6 -0.5792616277176208 2316 7 -0.4710848963309928 2316 8 -0.46811672025899753 2316 9 -0.6273300907624204 2316 10 -0.6503178242813092 2316 11 -0.6928424739800221 2316 12 -0.6354237248820453 2316 13 -0.6675571579584769 2316 14 -0.8505377436315982 2316 15 -0.8278144232603779 2316 16 -0.8010183373351367 2316 17 -0.7508106800182759 2316 18 -0.5637915637135151 2316 19 -0.6930777874584066 2316 20 -0.6825610841731091 2316 21 -0.6190576648597101 2316 22 -0.7324457583620401 2316 23 -0.8447171948430494 2316 24 -0.8506196820874905 2316 25 -0.8270837614733608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87615.0 3 3.0 4 670.0 5 3527.0 6 4132.0 7 3814.0 8 3488.0 9 3329.0 10 3259.0 11 3187.0 12 3246.0 13 3219.0 14 3320.0 15 3266.0 16 3426.0 17 3652.0 18 4195.0 19 5029.0 20 6153.0 21 7634.0 22 9201.0 23 10872.0 24 12884.0 25 15087.0 26 16928.0 27 18696.0 28 21800.0 29 26001.0 30 33086.0 31 42613.0 32 56365.0 33 76146.0 34 106255.0 35 152873.0 36 265477.0 37 445318.0 38 1297355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49924622467698 19.65321613830969 12.036181215504929 24.8113564215084 2 13.642920719551274 19.603147637529457 44.26882549473143 22.485106148187835 3 19.180653565915 24.629480902926538 29.2758012725089 26.91406425864956 4 11.733478065143837 16.567423725367757 39.210497654763806 32.4886005547246 5 12.388224127072856 38.53819753861678 36.439506271427305 12.634072062883059 6 31.482832674863403 37.53655932197855 18.316070645254573 12.664537357903471 7 27.923443662332463 31.068243255267813 22.534231674554572 18.474081407845148 8 23.909863323763613 39.71627780825678 20.363317772703457 16.01054109527615 9 26.512937972917854 14.893220174151342 20.6810525139308 37.912789339 10 15.106299152610625 28.946593210291727 35.31616207128983 20.630945565807814 11 34.18338691067943 21.31125414529015 26.482870971721322 18.022487972309097 12 24.63702650721484 23.65197212241668 32.84236626390097 18.868635106467508 13 29.87261634196287 22.418442416076402 25.685463283074917 22.023477958885813 14 19.662707102432815 23.598238425105308 27.62618820490845 29.11286626755343 15 21.641517033339593 34.41783825618233 22.761419933443573 21.17922477703451 16 20.5549240827575 27.905437029241874 30.805859421557997 20.73377946644263 17 20.560993241084695 29.78672909132077 29.147726839075887 20.504550828518646 18 20.408234253153935 26.965787717318758 32.917265423281975 19.708712606245328 19 23.709039882784456 26.293660017663512 27.665644328523886 22.331655771028146 20 23.786839043947214 27.65981596070889 29.09657131087381 19.45677368447009 21 21.870157769019077 26.945332583416466 27.24377628833941 23.940733359225046 22 22.91927329876027 29.939456061425425 27.46543297759903 19.675837662215276 23 20.751443786312368 29.317752004029103 29.14935260026677 20.78145160939176 24 23.43745232923879 26.542462390039447 30.038681417664876 19.981403863056883 25 21.863976920114236 28.216868082756214 29.495200168593623 20.423954828535923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1036.0 1 1036.0 2 1683.5 3 2331.0 4 2331.0 5 2331.0 6 3453.5 7 4576.0 8 4576.0 9 4576.0 10 5556.0 11 6536.0 12 6536.0 13 6536.0 14 9193.0 15 11850.0 16 11850.0 17 11850.0 18 20927.5 19 30005.0 20 30005.0 21 30005.0 22 50979.5 23 71954.0 24 71954.0 25 71954.0 26 110275.5 27 148597.0 28 148597.0 29 148597.0 30 200054.0 31 251511.0 32 251511.0 33 251511.0 34 277081.0 35 302651.0 36 302651.0 37 302651.0 38 315526.0 39 328401.0 40 328401.0 41 328401.0 42 339893.0 43 351385.0 44 351385.0 45 351385.0 46 356200.5 47 361016.0 48 361016.0 49 361016.0 50 337897.0 51 314778.0 52 314778.0 53 314778.0 54 270607.0 55 226436.0 56 226436.0 57 226436.0 58 192137.0 59 157838.0 60 157838.0 61 157838.0 62 128819.0 63 99800.0 64 99800.0 65 99800.0 66 76620.5 67 53441.0 68 53441.0 69 53441.0 70 38818.5 71 24196.0 72 24196.0 73 24196.0 74 16865.5 75 9535.0 76 9535.0 77 9535.0 78 6480.0 79 3425.0 80 3425.0 81 3425.0 82 2294.0 83 1163.0 84 1163.0 85 1163.0 86 785.5 87 408.0 88 408.0 89 408.0 90 270.0 91 132.0 92 132.0 93 132.0 94 99.0 95 66.0 96 66.0 97 66.0 98 60.0 99 54.0 100 54.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34917037654159916 2 0.30013162651943215 3 0.32126714682418905 4 0.3112784420226259 5 0.2955353746723361 6 0.2527576606308591 7 0.3528618544030464 8 0.3238367049434317 9 0.26625688849673973 10 0.2408508349797204 11 0.2526490877525812 12 0.2507309669030057 13 0.2688988285348343 14 0.2597787067594941 15 0.3498941957301182 16 0.40450635350388203 17 0.4214075315558023 18 0.11707775374295949 19 0.13607800744158507 20 0.19720453791202047 21 0.19839883957307697 22 0.14957723530746572 23 0.2595977519623643 24 0.35687905089932725 25 0.395965287079357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2763121.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.3420823736727 #Duplication Level Percentage of deduplicated Percentage of total 1 81.47971144426693 50.79614882641571 2 11.98960911746262 14.949143984579838 3 3.31138019912803 6.193150114337651 4 1.254991916527381 3.129552377537735 5 0.5988627907132213 1.8667176714585578 6 0.3220974656741621 1.2048136042445896 7 0.21983737968028794 0.9593584022988609 8 0.13896022196558216 0.6930455683553732 9 0.10003556816108321 0.5612783067535859 >10 0.5054732681673165 5.805690184932543 >50 0.040923041520604865 1.7696072081129155 >100 0.030252231433207384 3.8309408319563127 >500 0.004253118050883297 1.8162290940426946 >1k 0.0032044040108521266 4.0609813231811085 >5k 2.330475644256092E-4 1.0535828599673935 >10k+ 1.747856733192069E-4 1.309759641825222 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 13051 0.4723282114681189 No Hit CTGTAGGACGTGGAATATGGCAAGA 11715 0.42397708967504505 No Hit GTCCTAAAGTGTGTATTTCTCATTT 11294 0.4087406957567186 No Hit CTTTAGGACGTGAAATATGGCGAGG 9532 0.34497222524818855 No Hit GTCCTACAGTGTGCATTTCTCATTT 7079 0.25619580177632467 No Hit GTATCAACGCAGAGTACTTTTTTTT 6822 0.24689472520385464 No Hit CTGAAGGACCTGGAATATGGCGAGA 5574 0.20172840784026472 No Hit CTGTAGGACCTGGAATATGGCGAGA 4956 0.17936239491502542 No Hit GTCCTTCAGTGTGCATTTCTCATTT 4500 0.16285931741679063 No Hit GGTATCAACGCAGAGTACTTTTTTT 4069 0.1472610139042047 No Hit ATTTAGAAATGTCCACTGTAGGACG 3913 0.14161522423375597 No Hit TATCAACGCAGAGTACTTTTTTTTT 3739 0.13531799729364005 No Hit GAATATGGCAAGAAAACTGAAAATC 3639 0.1316989013510447 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3513 0.12713884046337456 No Hit GGAATATGGCGAGAAAACTGAAAAT 3368 0.12189115134661133 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 3073 0.11121481831595503 No Hit ACCTGGAATATGGCGAGAAAACTGA 2861 0.1035423349176529 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 2764 0.10003181185333541 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6190959425953476E-5 2 0.0 0.0 0.0 0.0 3.6190959425953476E-5 3 0.0 0.0 0.0 0.0 3.6190959425953476E-5 4 0.0 0.0 0.0 0.0 3.6190959425953476E-5 5 0.0 0.0 0.0 0.0 7.238191885190695E-5 6 0.0 0.0 0.0 0.0 7.238191885190695E-5 7 0.0 0.0 0.0 0.0 7.238191885190695E-5 8 0.0 0.0 0.0 0.0 7.238191885190695E-5 9 0.0 0.0 0.0 1.0857287827786044E-4 7.238191885190695E-5 10 0.0 0.0 0.0 1.0857287827786044E-4 7.238191885190695E-5 11 0.0 0.0 0.0 1.447638377038139E-4 7.238191885190695E-5 12 0.0 0.0 0.0 1.809547971297674E-4 1.0857287827786044E-4 13 3.6190959425953476E-5 0.0 0.0 1.809547971297674E-4 1.0857287827786044E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGT 55 1.9585634E-4 13.820219 6 TAGGACC 1800 0.0 13.566135 4 GGTATCA 1400 0.0 12.690926 1 CACCTTT 1330 0.0 12.011603 14 ACCTTTT 1375 0.0 11.895126 15 CCACCTT 1345 0.0 11.655993 13 GATATAC 1000 0.0 11.30646 1 AATCCCG 530 0.0 11.30049 19 TCCAACG 365 0.0 11.195467 18 TGTAGGA 6350 0.0 11.178052 2 ACTGTTC 1095 0.0 11.169658 8 TACTACG 60 0.0058679814 11.085774 2 ATAGGAC 490 0.0 11.053454 3 GTATTAG 215 0.0 11.047938 1 AGGACCT 3800 0.0 11.026826 5 TAGGACA 1590 0.0 10.935774 4 AAGACCG 70 0.0014899744 10.858941 5 GGACGGG 140 8.341885E-9 10.858744 6 CTGTAGG 5975 0.0 10.797212 1 GTGTAGG 625 0.0 10.793394 1 >>END_MODULE