FastQCFastQC Report
Thu 26 May 2016
SRR1512320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763121
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT133360.48264263490451553No Hit
CTGTAGGACGTGGAATATGGCAAGA127170.4602404310198504No Hit
GTCCTAAAGTGTGTATTTCTCATTT114440.4141693396706116No Hit
CTTTAGGACGTGAAATATGGCGAGG104580.37848505367662144No Hit
GTATCAACGCAGAGTACTTTTTTTT82090.2970915859276521No Hit
GTCCTACAGTGTGCATTTCTCATTT72430.262131119122181No Hit
CTGAAGGACCTGGAATATGGCGAGA61270.22174200840281696No Hit
CTGTAGGACCTGGAATATGGCGAGA57010.20632465968736077No Hit
GGTATCAACGCAGAGTACTTTTTTT51610.1867815415973459No Hit
TATCAACGCAGAGTACTTTTTTTTT50710.1835243552490101No Hit
GTCCTTCAGTGTGCATTTCTCATTT46310.16760033310159056No Hit
ATTTAGAAATGTCCACTGTAGGACG42670.1544268238705435No Hit
TTTCTAAATTTTCCACCTTTTTCAG41120.1488172251595207No Hit
GAATATGGCAAGAAAACTGAAAATC36840.13332749452521261No Hit
GGAATATGGCGAGAAAACTGAAAAT35780.12949125282606155No Hit
ATTCCAGGTCCTTCAGTGTGCATTT34360.12435213658757616No Hit
ACCTGGAATATGGCGAGAAAACTGA31680.11465295946142062No Hit
TTCCAGGTCCTTCAGTGTGCATTTC30790.11143196407251077No Hit
GACCTGGAATATGGCGAGAAAACTG29650.10730619469795205No Hit
ACAGTGGACATTTCTAAATTTTCCA28960.10480901849756127No Hit
GCCATATTCCACGTCCTACAGTGGA28200.10205850558118881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG456.7507126E-414.7818084
GGTATCA15150.014.3154491
AGCGTAC1152.5465852E-1114.0615711
TAGGACC18850.013.2078454
CCAACGA1900.012.49209419
GACTGCG550.003058654312.0966177
GGCGAGG27050.012.0736919
CCTATAC1950.011.6952843
TCCAACG3600.011.60397818
AGGACCT36550.011.2837175
ACCTTTT13700.011.22667615
CCGCTCA600.00586174811.087369
TGTAGGA66950.010.9994142
TAAGCGA951.3633178E-510.99841613
GCGTACA1658.0035534E-1110.9417832
GTATCAA34150.010.89102651
CTGTAGG65950.010.8752511
CCACCTT13900.010.86534313
GGACCTG36700.010.8252716
TACACGG1951.8189894E-1210.72106555
Produced by FastQC (version 0.11.5)