FastQCFastQC Report
Thu 26 May 2016
SRR1512319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342837
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67770.28926468209269357No Hit
GTCCTACAGTGGACATTTCTAAATT56640.2417581760916359No Hit
GTCCTAAAGTGTGTATTTCTCATTT50580.21589210004793336No Hit
CTGTAGGACGTGGAATATGGCAAGA49560.21153840408018143No Hit
CTTTAGGACGTGAAATATGGCGAGG42750.18247108100136714No Hit
GGTATCAACGCAGAGTACTTTTTTT41280.1761966368125482No Hit
TATCAACGCAGAGTACTTTTTTTTT38710.16522703030556543No Hit
GTCCTACAGTGTGCATTTCTCATTT28350.12100713792722241No Hit
CTGAAGGACCTGGAATATGGCGAGA23460.10013500725829412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.014.0047791
TAGGACC8800.013.2808524
CGTGCGC550.003091208912.07939810
GTCTTAG2250.011.8231311
GTCCTAG2900.011.7940081
CTAGGAC3800.011.5021173
TCCAACG1158.791194E-710.74202818
TACTATA1951.8189894E-1210.7196912
TGTAGGA29100.010.7096442
CTAATTC1355.6757926E-810.5574993
TCTAGGA4800.010.4912892
GTATAGG3000.010.4508021
GTCCTAA15050.010.4160821
GTATCAA28850.010.3734131
AGGACGT31150.010.3708685
GGCGAGG13900.010.25786719
GGACGTG30400.010.2512276
AACAGTG5200.010.2322147
GACGTGA15750.010.195097
CTAATAC2151.8189894E-1210.164663
Produced by FastQC (version 0.11.5)