##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512319_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2342837 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.98544755781132 31.0 30.0 34.0 16.0 34.0 2 29.88565102907287 31.0 30.0 34.0 22.0 34.0 3 30.57660648179963 33.0 31.0 34.0 26.0 34.0 4 34.233121638423846 37.0 35.0 37.0 31.0 37.0 5 34.40629288337174 37.0 35.0 37.0 32.0 37.0 6 34.55700033762486 37.0 35.0 37.0 32.0 37.0 7 34.49113617379271 37.0 35.0 37.0 32.0 37.0 8 34.48975750340293 37.0 35.0 37.0 32.0 37.0 9 36.069035959394526 39.0 37.0 39.0 32.0 39.0 10 36.00381503279998 39.0 37.0 39.0 32.0 39.0 11 36.02912579919132 39.0 37.0 39.0 32.0 39.0 12 35.962578702658355 39.0 37.0 39.0 32.0 39.0 13 35.94067662411 39.0 37.0 39.0 32.0 39.0 14 37.17690261849202 40.0 38.0 41.0 31.0 41.0 15 37.10710305497139 40.0 38.0 41.0 31.0 41.0 16 37.03670805950222 40.0 38.0 41.0 31.0 41.0 17 36.99040692971811 40.0 38.0 41.0 31.0 41.0 18 36.77220609030846 40.0 38.0 41.0 30.0 41.0 19 36.87563667468117 40.0 38.0 41.0 31.0 41.0 20 36.80349721299433 40.0 38.0 41.0 30.0 41.0 21 36.75430044855874 40.0 38.0 41.0 30.0 41.0 22 36.68117841744859 40.0 38.0 41.0 30.0 41.0 23 36.5566977130718 40.0 37.0 41.0 30.0 41.0 24 36.42861197770054 40.0 37.0 41.0 30.0 41.0 25 36.310041629016446 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.043665618487804 1101 2 0.26979365507279596 1101 3 -0.2143426940775761 1101 4 -0.3603719052877068 1101 5 -0.4845005844613297 1101 6 -0.4430008388258386 1101 7 -0.6047705543344648 1101 8 -0.6255472101368156 1101 9 -0.6892956683795006 1101 10 -0.8522253945193725 1101 11 -0.8962234905901454 1101 12 -0.8340256402711361 1101 13 -0.8248835667994996 1101 14 -1.2624300803224457 1101 15 -1.247015946115603 1101 16 -1.3998486484705879 1101 17 -1.4208115604779863 1101 18 -1.2537959369484 1101 19 -1.3762584483094713 1101 20 -1.5018260472782359 1101 21 -1.5675038855626866 1101 22 -1.5181035515980597 1101 23 -1.7940772116121693 1101 24 -1.9670188190377687 1101 25 -1.9413367712893148 1102 1 1.186702662161995 1102 2 0.31551254644212534 1102 3 -0.13453570693731365 1102 4 -0.13192183014552228 1102 5 -0.22963566416215286 1102 6 -0.36510945718210763 1102 7 -0.34621413357390907 1102 8 -0.4709912666851892 1102 9 -0.5210094494857955 1102 10 -0.46344389542112197 1102 11 -0.5087930220587751 1102 12 -0.8735119131805789 1102 13 -0.7809248582683637 1102 14 -0.8930074075538172 1102 15 -0.9633993203075448 1102 16 -1.0151701376914275 1102 17 -1.0716791883587717 1102 18 -1.1011509278205054 1102 19 -1.1440448941797072 1102 20 -1.333647265358728 1102 21 -1.1257878070886775 1102 22 -1.255510175915596 1102 23 -1.2931975448451425 1102 24 -1.4835295039208702 1102 25 -1.5053866186481244 1103 1 1.424813381231072 1103 2 0.5373710386829487 1103 3 -0.04273796335010971 1103 4 0.004568039520748357 1103 5 -0.07372443735327039 1103 6 -0.22707476129580328 1103 7 -0.34568235071144926 1103 8 -0.3123733989008244 1103 9 -0.433730562223964 1103 10 -0.40895182793202167 1103 11 -0.44425339365179184 1103 12 -0.5082695446041399 1103 13 -0.7057599566561805 1103 14 -0.9540410232998582 1103 15 -0.9577076661033956 1103 16 -0.9057498728703521 1103 17 -0.9190381365989069 1103 18 -0.7770557324976366 1103 19 -0.9762157803959539 1103 20 -0.9448247300664576 1103 21 -1.0822373267567542 1103 22 -1.274749812290338 1103 23 -1.1711331090571733 1103 24 -0.9813681250955781 1103 25 -1.2672351621761422 1104 1 1.496586931095301 1104 2 0.7884735111035504 1104 3 0.23934116064966204 1104 4 0.18989482272734648 1104 5 0.039531621824508534 1104 6 -0.06962892285587685 1104 7 0.049085862946697034 1104 8 0.07348073060322946 1104 9 -0.08475340793942365 1104 10 -0.04431646614576579 1104 11 -0.08482570964432057 1104 12 -0.059520585093970624 1104 13 -0.08405772478884899 1104 14 -0.25497730254242157 1104 15 -0.26366168263182743 1104 16 -0.48187893505865986 1104 17 -0.3167039409598189 1104 18 -0.28538725642120255 1104 19 -0.2628745807249544 1104 20 -0.32071582476633864 1104 21 -0.31590177890867466 1104 22 -0.3919436668122742 1104 23 -0.4831205406624335 1104 24 -0.42202721670469145 1104 25 -0.6302329289018402 1105 1 -0.6686802923316613 1105 2 -0.8562260512582291 1105 3 -0.907838895689121 1105 4 -0.5956435301534313 1105 5 -0.4618649775210031 1105 6 -0.23580755254895536 1105 7 -0.1533828350517723 1105 8 -0.12176534166976438 1105 9 -0.2212614421878243 1105 10 -0.29385705358549785 1105 11 -0.17349214830485948 1105 12 -0.26758829252700167 1105 13 -0.3267785302983839 1105 14 -0.6054220999594762 1105 15 -0.5115311731123242 1105 16 -0.49059052486722976 1105 17 -0.4677158356091127 1105 18 -0.32055522299481254 1105 19 -0.33070961804550336 1105 20 -0.44914138806140613 1105 21 -0.4734418945092642 1105 22 -0.4754565982464811 1105 23 -0.540494993243712 1105 24 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-0.4555497468049907 2314 7 -0.5368112892767201 2314 8 -0.5578534647187965 2314 9 -0.6279743943148972 2314 10 -0.48900157656849785 2314 11 -1.545466675697952 2314 12 -1.0415962060595803 2314 13 -0.9216075713861756 2314 14 -0.8190800885129406 2314 15 -0.7830054381171152 2314 16 -1.0889140730468938 2314 17 -1.2072948317336554 2314 18 -2.2103302712225243 2314 19 -1.8931466386941054 2314 20 -2.2084426221487305 2314 21 -1.6640284986626384 2314 22 -1.1973256159780163 2314 23 -0.9351581014487067 2314 24 -0.8979021434644991 2314 25 -0.9607492444688077 2315 1 -2.7665003239995833 2315 2 -1.2939276312403756 2315 3 -0.5831540333318408 2315 4 -0.4633452662786013 2315 5 -0.2788488130129494 2315 6 -0.24179034452912873 2315 7 -0.24612729217291474 2315 8 -0.33539665411539943 2315 9 -0.3609140424852413 2315 10 -0.4112616548292962 2315 11 -0.5928937658936562 2315 12 -0.557822710842629 2315 13 -0.4810398983706321 2315 14 -0.5552739890386462 2315 15 -1.0694430327572135 2315 16 -0.8335255955695828 2315 17 -0.7161408420996338 2315 18 -0.4841192748480623 2315 19 -0.5633201150381595 2315 20 -0.5666502610872257 2315 21 -0.5647501004603228 2315 22 -0.5111631996824144 2315 23 -0.5764077564009469 2315 24 -0.4273378040733178 2315 25 -0.2434052038515091 2316 1 1.3953443615554662 2316 2 0.5389760846479135 2316 3 -0.006389910634318596 2316 4 -0.047583318787928874 2316 5 -0.28528526682239885 2316 6 -0.36107301137099057 2316 7 -0.30450315065704814 2316 8 -0.34625864347286495 2316 9 -0.5112175929206799 2316 10 -0.5266421441635245 2316 11 -0.6288527321839155 2316 12 -0.5529549451425595 2316 13 -0.5167725751409549 2316 14 -0.6455637846933939 2316 15 -0.6357163400464643 2316 16 -0.6102029724111162 2316 17 -0.4967911754493244 2316 18 -0.4030358313036899 2316 19 -0.5109792289571473 2316 20 -0.4925558280404161 2316 21 -0.4726117347215748 2316 22 -0.6094962987797601 2316 23 -0.5803835773334001 2316 24 -0.6970356414976493 2316 25 -0.7151512155333677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65043.0 3 4.0 4 544.0 5 2531.0 6 3162.0 7 2972.0 8 2698.0 9 2658.0 10 2478.0 11 2575.0 12 2627.0 13 2657.0 14 2662.0 15 2702.0 16 2811.0 17 2896.0 18 3426.0 19 3972.0 20 4937.0 21 5969.0 22 7322.0 23 8874.0 24 10372.0 25 12309.0 26 13690.0 27 15196.0 28 17737.0 29 21492.0 30 26760.0 31 34637.0 32 46313.0 33 62757.0 34 88227.0 35 128115.0 36 222146.0 37 374806.0 38 1132760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.575722818652245 19.019469611764173 11.814625379794057 24.590182189789527 2 14.910674993279006 19.763917594620466 41.66695205919269 23.658455352907836 3 18.96668365096832 24.622370466154855 29.345876026015954 27.065069856860873 4 12.40300685181348 16.40534958633953 38.104585628830705 33.08705793301629 5 13.220326939178765 37.851891107028315 35.563582256178265 13.364199697614648 6 32.05553354288634 36.64283593163654 18.097352289522036 13.204278235955085 7 28.20723796927041 31.29073349415425 22.24344323524171 18.25858530133363 8 25.228469018860284 36.97749072229309 20.388304201740404 17.405736057106232 9 26.90267024319601 14.878059635830798 19.906912093724696 38.3123580272485 10 15.477334433161886 28.373822483222337 34.349220094346805 21.799622989268972 11 34.74949882649448 21.705808477908786 24.928680389300027 18.61601230629671 12 24.133634452145472 23.961024568453894 31.645159606377582 20.26018137302305 13 29.573984391182506 21.71419669712081 25.638141800919957 23.07367711077673 14 21.151746876718367 21.962227495148458 27.269805745682824 29.616219882450356 15 23.012278291960968 31.18738411604393 23.128178309564408 22.672159282430695 16 22.377853429257584 27.44374563961212 27.92255265462985 22.255848276500444 17 21.963361893454604 28.37353038245417 27.738835621429093 21.924272102662133 18 21.901248991008924 26.71936566349416 30.03975285968048 21.339632485816438 19 23.986634915045038 26.178719119525024 27.369875050644747 22.46477091478519 20 24.12188231550911 26.779991395656943 27.92742245186144 21.170703836972503 21 23.21682029965382 26.212802535104657 26.986377233665404 23.583999931576123 22 23.77033203215167 27.747015061904445 27.024696804154207 21.45795610178968 23 22.47379341892488 27.489684040441386 27.864195201105048 22.172327339528685 24 23.726865733014883 26.13970852793931 28.353462130220098 21.779963608825717 25 23.04657186748111 26.876361836650304 28.063958383771727 22.013107912096856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 972.0 1 972.0 2 1480.0 3 1988.0 4 1988.0 5 1988.0 6 3101.0 7 4214.0 8 4214.0 9 4214.0 10 4979.0 11 5744.0 12 5744.0 13 5744.0 14 7897.5 15 10051.0 16 10051.0 17 10051.0 18 17320.0 19 24589.0 20 24589.0 21 24589.0 22 39521.5 23 54454.0 24 54454.0 25 54454.0 26 80335.0 27 106216.0 28 106216.0 29 106216.0 30 139648.5 31 173081.0 32 173081.0 33 173081.0 34 194825.0 35 216569.0 36 216569.0 37 216569.0 38 232874.0 39 249179.0 40 249179.0 41 249179.0 42 265188.5 43 281198.0 44 281198.0 45 281198.0 46 291928.0 47 302658.0 48 302658.0 49 302658.0 50 295319.0 51 287980.0 52 287980.0 53 287980.0 54 258403.5 55 228827.0 56 228827.0 57 228827.0 58 198864.0 59 168901.0 60 168901.0 61 168901.0 62 140503.0 63 112105.0 64 112105.0 65 112105.0 66 87593.0 67 63081.0 68 63081.0 69 63081.0 70 46847.0 71 30613.0 72 30613.0 73 30613.0 74 21731.0 75 12849.0 76 12849.0 77 12849.0 78 8948.5 79 5048.0 80 5048.0 81 5048.0 82 3351.5 83 1655.0 84 1655.0 85 1655.0 86 1100.5 87 546.0 88 546.0 89 546.0 90 375.0 91 204.0 92 204.0 93 204.0 94 135.0 95 66.0 96 66.0 97 66.0 98 57.5 99 49.0 100 49.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3422773329941434 2 0.2933622782976366 3 0.31359415955954256 4 0.30326480245958215 5 0.28789881669104594 6 0.247520420754837 7 0.3478261611883371 8 0.3158563741310215 9 0.2612644413589165 10 0.23488616578959612 11 0.24850213651227124 12 0.24649602170360124 13 0.26502057121344763 14 0.2540509647064649 15 0.3442834478028134 16 0.39819244787409447 17 0.41603406468311716 18 0.1121716961103141 19 0.1284340310486816 20 0.19134920611207695 21 0.19087968988026055 22 0.14619028127010117 23 0.25285583248002313 24 0.35021642564122046 25 0.3897838389952011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2342837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.45229658948867 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75616248425891 56.64849378980406 2 11.317669757528034 15.494409738883908 3 2.9529810753173806 6.0641500917231745 4 1.182155395009853 3.2368500685631436 5 0.5668436454010953 1.9400874667431363 6 0.29783970093216167 1.223268692659976 7 0.20834080607876224 0.9982984654577605 8 0.13239121363543885 0.7249986097292334 9 0.09621245850094756 0.5927367370419659 >10 0.4425109628509234 5.220130080388887 >50 0.024986622101198872 1.2082467991604258 >100 0.01752913934803126 2.4409486122127086 >500 0.0025635185790124893 1.236868277138636 >1k 0.001625645928150883 2.221560773489043 >5k 1.8757453017125572E-4 0.7489517970039062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6777 0.28926468209269357 No Hit GTCCTACAGTGGACATTTCTAAATT 5664 0.2417581760916359 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5058 0.21589210004793336 No Hit CTGTAGGACGTGGAATATGGCAAGA 4956 0.21153840408018143 No Hit CTTTAGGACGTGAAATATGGCGAGG 4275 0.18247108100136714 No Hit GGTATCAACGCAGAGTACTTTTTTT 4128 0.1761966368125482 No Hit TATCAACGCAGAGTACTTTTTTTTT 3871 0.16522703030556543 No Hit GTCCTACAGTGTGCATTTCTCATTT 2835 0.12100713792722241 No Hit CTGAAGGACCTGGAATATGGCGAGA 2346 0.10013500725829412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.268329380148939E-5 0.0 6 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 0.0 7 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 0.0 8 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 0.0 9 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 4.268329380148939E-5 10 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 4.268329380148939E-5 11 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 4.268329380148939E-5 12 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 8.536658760297878E-5 13 1.7073317520595755E-4 0.0 0.0 4.268329380148939E-5 8.536658760297878E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1350 0.0 14.004779 1 TAGGACC 880 0.0 13.280852 4 CGTGCGC 55 0.0030912089 12.079398 10 GTCTTAG 225 0.0 11.823131 1 GTCCTAG 290 0.0 11.794008 1 CTAGGAC 380 0.0 11.502117 3 TCCAACG 115 8.791194E-7 10.742028 18 TACTATA 195 1.8189894E-12 10.719691 2 TGTAGGA 2910 0.0 10.709644 2 CTAATTC 135 5.6757926E-8 10.557499 3 TCTAGGA 480 0.0 10.491289 2 GTATAGG 300 0.0 10.450802 1 GTCCTAA 1505 0.0 10.416082 1 GTATCAA 2885 0.0 10.373413 1 AGGACGT 3115 0.0 10.370868 5 GGCGAGG 1390 0.0 10.257867 19 GGACGTG 3040 0.0 10.251227 6 AACAGTG 520 0.0 10.232214 7 GACGTGA 1575 0.0 10.19509 7 CTAATAC 215 1.8189894E-12 10.16466 3 >>END_MODULE