FastQCFastQC Report
Thu 26 May 2016
SRR1512319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342837
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78240.333954090702853No Hit
CTGTAGGACGTGGAATATGGCAAGA55720.23783131306189886No Hit
GTCCTACAGTGGACATTTCTAAATT55540.23706301377347205No Hit
GTCCTAAAGTGTGTATTTCTCATTT51010.21772748168139738No Hit
GGTATCAACGCAGAGTACTTTTTTT48380.20650177541160566No Hit
TATCAACGCAGAGTACTTTTTTTTT47820.20411151095872226No Hit
CTTTAGGACGTGAAATATGGCGAGG46960.20044074769179418No Hit
GTCCTACAGTGTGCATTTCTCATTT30380.12967184656892478No Hit
CTGAAGGACCTGGAATATGGCGAGA27270.11639734219666158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.013.0269611
TACCGAC604.079527E-412.6717667
TAGGACC8600.011.8225054
GATATAC4400.011.67351151
TCTATAC2500.011.402643
TCCAACG1803.6379788E-1211.07690818
CCAACGA1502.1536835E-910.76065619
GGCGAGG11850.010.73661819
GTATCAA29900.010.7206021
CTAGGAC4000.010.6899763
TAGACTG2550.010.4342355
AGGACCT18050.010.4239115
TGTAGGA28550.010.3176252
GGACCTG17450.010.2390786
GTATTAT2550.010.0712651
ATTGCGA856.630856E-410.05299317
TCGAACT1803.9835868E-1010.02217919
GCCTCGA1803.9835868E-1010.02196516
GTAGGAC28450.010.0198953
AGGACGT29900.010.0111075
Produced by FastQC (version 0.11.5)