FastQCFastQC Report
Thu 26 May 2016
SRR1512318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008669
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72280.35984027233954424No Hit
GTATCAACGCAGAGTACTTTTTTTT72050.3586952355017178No Hit
CTGTAGGACGTGGAATATGGCAAGA67570.3363919092692723No Hit
GTCCTAAAGTGTGTATTTCTCATTT64970.3234480145807995No Hit
CTTTAGGACGTGAAATATGGCGAGG54990.27376337266120004No Hit
GGTATCAACGCAGAGTACTTTTTTT43910.21860246760416974No Hit
TATCAACGCAGAGTACTTTTTTTTT41230.20526029923297467No Hit
GTCCTACAGTGTGCATTTCTCATTT37380.18609337825196687No Hit
CTGAAGGACCTGGAATATGGCGAGA31120.15492846257895154No Hit
ATTTAGAAATGTCCACTGTAGGACG26420.1315298837190199No Hit
GTCCTTCAGTGTGCATTTCTCATTT26370.1312809626673185No Hit
CTGTAGGACCTGGAATATGGCGAGA25780.12834369425724199No Hit
TTTCTAAATTTTCCACCTTTTTCAG22100.11002310485201892No Hit
GAATATGGCAAGAAAACTGAAAATC21490.10698626802126185No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20560.10235633645961578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1203.6379788E-1214.25880519
GGTATCA11050.014.099791
TGCGATA751.4941099E-512.65422110
TAGGACC11350.012.555914
CTAGGAC2950.012.2387223
TAGAAAT9100.012.1107034
CGATAAG802.883747E-511.86837412
CACCTTT9800.011.83858114
AAATGTC9050.011.7576967
AATGTCC9200.011.7507738
ACCTTTT10150.011.71142915
CCACCTT9500.011.70283513
AGGACCT22500.011.5693775
GGCGAGG14150.011.42048119
TATTGCG752.0548509E-411.41161616
GTCGAGG1251.8158971E-811.40704419
ATTTAGA9350.011.2782691
TTCCACC9350.011.26924611
GTGTAGG3550.011.239631
GTATTGC855.3234675E-511.17666151
Produced by FastQC (version 0.11.5)