FastQCFastQC Report
Thu 26 May 2016
SRR1512318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008669
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82240.40942534583846313No Hit
GTCCTACAGTGGACATTTCTAAATT74840.37258503018665595No Hit
CTGTAGGACGTGGAATATGGCAAGA72650.3616822881221346No Hit
GTCCTAAAGTGTGTATTTCTCATTT64770.32245233037399396No Hit
CTTTAGGACGTGAAATATGGCGAGG61130.30433087781013196No Hit
TATCAACGCAGAGTACTTTTTTTTT53390.2657978990067552No Hit
GGTATCAACGCAGAGTACTTTTTTT52190.25982379376592163No Hit
GTCCTACAGTGTGCATTTCTCATTT37850.18843323613796No Hit
CTGAAGGACCTGGAATATGGCGAGA34360.17105854672920226No Hit
CTGTAGGACCTGGAATATGGCGAGA28990.1443244257764719No Hit
GTCCTTCAGTGTGCATTTCTCATTT27680.1378026942218952No Hit
ATTTAGAAATGTCCACTGTAGGACG27650.13765334159087436No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26160.13023549425017264No Hit
TTTCTAAATTTTCCACCTTTTTCAG24930.12411203637831818No Hit
GAATATGGCAAGAAAACTGAAAATC23110.11505131009638722No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21140.10524382065935205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT350.002164041416.2929786
CCACGAC400.005272055514.2531593
CCAACGA852.7209717E-713.40338319
CGAGACA500.0014970313.3032794
TAGGACC11500.013.13802054
GGTATCA13400.012.9167871
GTCGAGG1302.0008883E-1012.41531419
TTCGCAT550.00308338512.08335317
TCCAACG1552.5465852E-1111.63788418
CCACCTT8950.011.24940613
TTATACC1451.2132659E-911.1406774
CACCTTT9100.010.95523414
ACCTTTT9250.010.87995315
TAAGACG700.001488665810.8598194
GTGGTAT5100.010.8154781
TGTAGGA39500.010.6808472
GGCGAGG15500.010.65785119
ATAGGAC2950.010.6294743
GTCCTAA18250.010.5784261
AGGACGT41450.010.5230215
Produced by FastQC (version 0.11.5)