FastQCFastQC Report
Thu 26 May 2016
SRR1512317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889307
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75440.3992998490981085No Hit
GTCCTAAAGTGTGTATTTCTCATTT48410.25623151769405395No Hit
GTCCTACAGTGGACATTTCTAAATT47950.2537967625166265No Hit
GGTATCAACGCAGAGTACTTTTTTT46950.24850381647874062No Hit
CTGTAGGACGTGGAATATGGCAAGA46750.24744522727116344No Hit
TATCAACGCAGAGTACTTTTTTTTT44850.23738862979918035No Hit
CTTTAGGACGTGAAATATGGCGAGG42830.22669687880265094No Hit
GTCCTACAGTGTGCATTTCTCATTT23600.12491352649410603No Hit
CTGAAGGACCTGGAATATGGCGAGA22250.11776804934296015No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21620.11443349333909207No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20990.11109893733522397No Hit
GTCCTTCAGTGTGCATTTCTCATTT19750.10453568424824553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.012.885831
TAGGACC6000.012.5079484
TCCAACG1551.8189894E-1212.26094718
GGCGAGG12350.011.23669819
AGAGTCG600.00587778111.0829935
CGAATGG1303.241439E-810.96732619
ACACCGA700.001492368910.856526
TCCGAAT1158.7077206E-710.74966417
CCGAATG1158.791685E-710.74165718
GTGGTAT4250.010.727941
AAGTCGT2450.010.4687877
TGTAGGA26600.010.4642422
GTACTAG1002.4027426E-510.4485661
ATTTCTA17150.010.42337615
GTATCAA27850.010.4025231
TAGAAAT6500.010.3766034
TTAGGAC17000.010.3381923
GTCTAAA1201.5265487E-610.2902551
AGGACGT30850.010.1925545
CCAACGA1409.614632E-810.18394619
Produced by FastQC (version 0.11.5)