##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1889307 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.91927410420858 31.0 30.0 34.0 16.0 34.0 2 29.82991276695635 31.0 30.0 34.0 22.0 34.0 3 30.502771651192738 33.0 31.0 34.0 26.0 34.0 4 34.164597918707756 37.0 35.0 37.0 30.0 37.0 5 34.33803452800419 37.0 35.0 37.0 31.0 37.0 6 34.49426800408827 37.0 35.0 37.0 32.0 37.0 7 34.43338589228749 37.0 35.0 37.0 32.0 37.0 8 34.43241834175176 37.0 35.0 37.0 32.0 37.0 9 36.00741912246131 39.0 37.0 39.0 32.0 39.0 10 35.93935395359251 39.0 37.0 39.0 31.0 39.0 11 35.95634589825793 39.0 37.0 39.0 31.0 39.0 12 35.904481378621895 39.0 37.0 39.0 31.0 39.0 13 35.87711525972222 39.0 37.0 39.0 31.0 39.0 14 37.097003822036335 40.0 38.0 41.0 31.0 41.0 15 37.03211336220106 40.0 38.0 41.0 31.0 41.0 16 36.960833787203455 40.0 38.0 41.0 31.0 41.0 17 36.920117270512414 40.0 38.0 41.0 31.0 41.0 18 36.71149156807231 40.0 38.0 41.0 30.0 41.0 19 36.814607154898596 40.0 38.0 41.0 30.0 41.0 20 36.749956465518835 40.0 38.0 41.0 30.0 41.0 21 36.694927822741356 40.0 38.0 41.0 30.0 41.0 22 36.61826690950703 40.0 38.0 41.0 30.0 41.0 23 36.500120414522364 40.0 37.0 41.0 30.0 41.0 24 36.37341893085666 40.0 37.0 41.0 30.0 41.0 25 36.25187806957789 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1001508048152289 1101 2 0.2092479930861728 1101 3 -0.12588422050530923 1101 4 -0.3061146881057084 1101 5 -0.5596340338279759 1101 6 -0.5910323939599778 1101 7 -0.5619045541299883 1101 8 -0.6511492679513182 1101 9 -0.8882925605638121 1101 10 -0.7223707294713648 1101 11 -0.781596021530298 1101 12 -0.8288529792823311 1101 13 -1.1206662828389184 1101 14 -1.2974810416966065 1101 15 -1.2475086991255608 1101 16 -1.3924553127702524 1101 17 -1.1856527633679619 1101 18 -1.360537436747201 1101 19 -1.3660581434001884 1101 20 -1.4454779310124906 1101 21 -1.5607123093919881 1101 22 -1.5890636155567108 1101 23 -1.5324499415369388 1101 24 -1.8258342871137287 1101 25 -1.9471629342959318 1102 1 0.9598613357366261 1102 2 0.0993450135206011 1102 3 -0.4605724800195077 1102 4 -0.39975197406005236 1102 5 -0.5746858206067245 1102 6 -0.6139610872961896 1102 7 -0.7020769091618817 1102 8 -0.6778486388251892 1102 9 -0.7089212129759019 1102 10 -0.670995165777768 1102 11 -0.9424482170239017 1102 12 -1.0962638245694123 1102 13 -1.1291445502879256 1102 14 -1.28922318551529 1102 15 -1.4411021660039935 1102 16 -1.5116833338191995 1102 17 -1.4963517236847181 1102 18 -1.42597129165474 1102 19 -1.4040886272654305 1102 20 -1.5354458628179017 1102 21 -1.5376868982756235 1102 22 -1.6946544296046042 1102 23 -1.9493871328789183 1102 24 -1.8306820191146826 1102 25 -1.806385270708006 1103 1 1.1580487256444734 1103 2 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-0.6864740793354613 2315 23 -0.7977274165330854 2315 24 -0.6941508625914281 2315 25 -0.4726537507074653 2316 1 1.2295856974118244 2316 2 0.4863166459691399 2316 3 -0.08502567146744155 2316 4 -0.1914512141313267 2316 5 -0.2789951214996407 2316 6 -0.5671374137121532 2316 7 -0.5221258863051901 2316 8 -0.45917016826098234 2316 9 -0.6071517469228809 2316 10 -0.5986743360746374 2316 11 -0.6988586009074353 2316 12 -0.74444661180884 2316 13 -0.6232970804077098 2316 14 -0.7172603007607208 2316 15 -0.7335545819045421 2316 16 -0.6220026712564746 2316 17 -0.6806241860766775 2316 18 -0.5603054622643953 2316 19 -0.7260565870496478 2316 20 -0.5206607478591607 2316 21 -0.516235680082076 2316 22 -0.7940112887039632 2316 23 -0.8014696971899014 2316 24 -0.8177599578986374 2316 25 -0.8423925025216903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54163.0 3 4.0 4 398.0 5 2130.0 6 2678.0 7 2416.0 8 2324.0 9 2094.0 10 2109.0 11 2066.0 12 2120.0 13 2075.0 14 2179.0 15 2225.0 16 2348.0 17 2489.0 18 2761.0 19 3382.0 20 4028.0 21 4936.0 22 6059.0 23 7105.0 24 8559.0 25 10045.0 26 11122.0 27 12760.0 28 14418.0 29 17413.0 30 22176.0 31 28681.0 32 37965.0 33 51194.0 34 71660.0 35 103312.0 36 179548.0 37 301457.0 38 906908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83660743451576 19.672651849333807 12.181356080745523 24.30938463540491 2 14.49785016279728 20.242924436898736 42.197277492247686 23.0619479080563 3 18.5850275404345 25.22579545472648 29.8720656148927 26.31711138994632 4 12.155133507839503 17.068721096309353 38.74121938398968 32.034926011861465 5 12.822732926442773 38.13301042566373 35.94807500277377 13.096181645119728 6 31.053504091901416 37.4110485052965 18.52674567805847 13.008701724743613 7 27.43983282587587 31.760374548228288 22.84769689385038 17.95209573204546 8 24.603124873885683 37.3868688177433 20.939490614970595 17.070515693400424 9 26.63082214717906 14.862897631894722 20.682033000946266 37.82424721997995 10 15.397194451725321 28.64820496059307 34.691254919896814 21.263345667784797 11 33.74878148827973 22.10386893722429 25.621248615659248 18.526100958836725 12 23.805266014999212 24.470270347715758 32.19863740973065 19.52582622755438 13 29.202467533087884 22.29887339058641 25.972308984055932 22.526350092269773 14 20.561420714740983 22.865947987863873 28.03579701861337 28.536834278781782 15 22.617775962341863 31.904350371104968 23.557104696059376 21.920768970493796 16 21.459156531420813 28.008141855354516 28.813452130726734 21.719249482497936 17 21.252885605380474 28.89421847733587 28.384736044513403 21.46815987277025 18 21.13267039206802 26.86607897532915 31.20768185178414 20.79356878081869 19 23.451821308755953 26.51834028502833 28.11901443161281 21.910823974602906 20 23.657390319337143 27.05016185348759 28.804829725039987 20.487618102135276 21 22.646104498778598 26.53492326311803 27.74906206941906 23.06991016868431 22 23.20510435005256 28.357747677973443 27.82694573924494 20.61020223272906 23 21.780848959284086 27.989749914034274 28.770552175887993 21.458848950793644 24 23.119284574093697 26.521222938462643 29.32316608366998 21.036326403773675 25 22.386304518911473 27.392396937689405 28.937049611365858 21.28424893203326 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 964.0 1 964.0 2 1437.5 3 1911.0 4 1911.0 5 1911.0 6 3184.0 7 4457.0 8 4457.0 9 4457.0 10 5078.5 11 5700.0 12 5700.0 13 5700.0 14 7772.0 15 9844.0 16 9844.0 17 9844.0 18 16742.5 19 23641.0 20 23641.0 21 23641.0 22 37684.0 23 51727.0 24 51727.0 25 51727.0 26 75425.5 27 99124.0 28 99124.0 29 99124.0 30 127449.0 31 155774.0 32 155774.0 33 155774.0 34 172207.0 35 188640.0 36 188640.0 37 188640.0 38 199673.5 39 210707.0 40 210707.0 41 210707.0 42 219327.0 43 227947.0 44 227947.0 45 227947.0 46 235423.0 47 242899.0 48 242899.0 49 242899.0 50 232392.0 51 221885.0 52 221885.0 53 221885.0 54 195308.5 55 168732.0 56 168732.0 57 168732.0 58 144802.0 59 120872.0 60 120872.0 61 120872.0 62 99765.0 63 78658.0 64 78658.0 65 78658.0 66 60723.0 67 42788.0 68 42788.0 69 42788.0 70 31431.5 71 20075.0 72 20075.0 73 20075.0 74 14112.5 75 8150.0 76 8150.0 77 8150.0 78 5641.5 79 3133.0 80 3133.0 81 3133.0 82 2118.5 83 1104.0 84 1104.0 85 1104.0 86 724.0 87 344.0 88 344.0 89 344.0 90 238.0 91 132.0 92 132.0 93 132.0 94 95.5 95 59.0 96 59.0 97 59.0 98 49.5 99 40.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34944029742122373 2 0.3004276171104008 3 0.3215994012619442 4 0.31212502785412855 5 0.29587568351781895 6 0.2568666712186003 7 0.3556330442855502 8 0.3226050610091425 9 0.26771721059626624 10 0.24416360072767423 11 0.25596687039215965 12 0.25448484550155165 13 0.27301015663415207 14 0.26549417326035424 15 0.35388637209304785 16 0.4060748200266024 17 0.4226946705855639 18 0.11972643937697791 19 0.13110627335843247 20 0.19324545984321237 21 0.19970285400943308 22 0.15434230646475136 23 0.2559139409317808 24 0.3594968948932069 25 0.39485377442628433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1889307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.66110512748905 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62038070907857 57.41467750666701 2 11.102602305512008 15.246338881749248 3 2.7503146518999366 5.66518930343325 4 1.0233153017939096 2.8104783806015927 5 0.45390610131952 1.5582847270354128 6 0.24865015775589225 1.0243556772986433 7 0.16964792083583805 0.8153749609018591 8 0.11303158230583064 0.6208698684341656 9 0.08081077579655739 0.4993701455161238 >10 0.37955488269897786 4.654715944121929 >50 0.02901595782801886 1.4006707623124948 >100 0.023975743574252462 3.430919390996877 >500 0.0027062392907783745 1.3430591138512222 >1k 0.002010349187435363 3.1151878679169105 >5k 7.73211225936678E-5 0.400507469163318 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7544 0.3992998490981085 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4841 0.25623151769405395 No Hit GTCCTACAGTGGACATTTCTAAATT 4795 0.2537967625166265 No Hit GGTATCAACGCAGAGTACTTTTTTT 4695 0.24850381647874062 No Hit CTGTAGGACGTGGAATATGGCAAGA 4675 0.24744522727116344 No Hit TATCAACGCAGAGTACTTTTTTTTT 4485 0.23738862979918035 No Hit CTTTAGGACGTGAAATATGGCGAGG 4283 0.22669687880265094 No Hit GTCCTACAGTGTGCATTTCTCATTT 2360 0.12491352649410603 No Hit CTGAAGGACCTGGAATATGGCGAGA 2225 0.11776804934296015 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2162 0.11443349333909207 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2099 0.11109893733522397 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1975 0.10453568424824553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.292946037885849E-5 2 0.0 0.0 0.0 0.0 5.292946037885849E-5 3 0.0 0.0 0.0 0.0 5.292946037885849E-5 4 0.0 0.0 0.0 0.0 5.292946037885849E-5 5 0.0 0.0 0.0 0.0 5.292946037885849E-5 6 0.0 0.0 0.0 5.292946037885849E-5 5.292946037885849E-5 7 0.0 0.0 0.0 5.292946037885849E-5 5.292946037885849E-5 8 0.0 0.0 0.0 5.292946037885849E-5 5.292946037885849E-5 9 0.0 0.0 0.0 5.292946037885849E-5 5.292946037885849E-5 10 0.0 0.0 0.0 1.0585892075771698E-4 5.292946037885849E-5 11 0.0 0.0 0.0 1.0585892075771698E-4 5.292946037885849E-5 12 0.0 0.0 0.0 1.0585892075771698E-4 5.292946037885849E-5 13 0.0 0.0 0.0 1.0585892075771698E-4 5.292946037885849E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1290 0.0 12.88583 1 TAGGACC 600 0.0 12.507948 4 TCCAACG 155 1.8189894E-12 12.260947 18 GGCGAGG 1235 0.0 11.236698 19 AGAGTCG 60 0.005877781 11.082993 5 CGAATGG 130 3.241439E-8 10.967326 19 ACACCGA 70 0.0014923689 10.85652 6 TCCGAAT 115 8.7077206E-7 10.749664 17 CCGAATG 115 8.791685E-7 10.741657 18 GTGGTAT 425 0.0 10.72794 1 AAGTCGT 245 0.0 10.468787 7 TGTAGGA 2660 0.0 10.464242 2 GTACTAG 100 2.4027426E-5 10.448566 1 ATTTCTA 1715 0.0 10.423376 15 GTATCAA 2785 0.0 10.402523 1 TAGAAAT 650 0.0 10.376603 4 TTAGGAC 1700 0.0 10.338192 3 GTCTAAA 120 1.5265487E-6 10.290255 1 AGGACGT 3085 0.0 10.192554 5 CCAACGA 140 9.614632E-8 10.183946 19 >>END_MODULE