FastQCFastQC Report
Thu 26 May 2016
SRR1512317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889307
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90200.47742373261730364No Hit
GGTATCAACGCAGAGTACTTTTTTT59230.31350119382397884No Hit
TATCAACGCAGAGTACTTTTTTTTT56290.2979399324725945No Hit
CTGTAGGACGTGGAATATGGCAAGA50660.2681406462792971No Hit
GTCCTACAGTGGACATTTCTAAATT50500.2672937749132354No Hit
GTCCTAAAGTGTGTATTTCTCATTT48940.2590367790941335No Hit
CTTTAGGACGTGAAATATGGCGAGG45720.24199349285214103No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27520.14566187496261856No Hit
GTCCTACAGTGTGCATTTCTCATTT25030.1324824393282828No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23540.1245959497318329No Hit
CTGAAGGACCTGGAATATGGCGAGA23090.12221412401478425No Hit
TTTCTAAATTTTCCACCTTTTTCAG19730.1044298253274878No Hit
GTCCTTCAGTGTGCATTTCTCATTT19640.10395346018407807No Hit
ATTTAGAAATGTCCACTGTAGGACG19190.10157163446702945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.015.10858251
CAGTCCG602.5599034E-514.2552669
TAGGACC6450.012.5240064
GGACCGA550.003057958612.0966586
GTATTAC1156.9703674E-811.5777731
GCGCCAC855.3356103E-511.17408813
ACCGAGC600.00585569211.0886048
GTAAGAC1803.6379788E-1211.0871343
CGACTAA1553.1650416E-1011.02552717
CCAACGA951.3697469E-510.99328919
TAAGCGA1605.456968E-1010.68522213
TTAGACT1252.2290988E-710.6439314
CACCTTT7350.010.59301514
GGCGAGG12100.010.51424219
AATGTCC6700.010.4975268
TCGCGCG1106.0196835E-610.3674659
GTATCAA31600.010.3229041
AAGCGAC1752.382876E-1010.30881114
AAATGTC6850.010.1289027
TCCCGCC2450.010.07948313
Produced by FastQC (version 0.11.5)