FastQCFastQC Report
Thu 26 May 2016
SRR1512316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230769
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81560.3656138309255687No Hit
CTGTAGGACGTGGAATATGGCAAGA74840.3354896898782438No Hit
GTCCTAAAGTGTGTATTTCTCATTT74320.3331586551543436No Hit
GTATCAACGCAGAGTACTTTTTTTT69570.3118655495033327No Hit
CTTTAGGACGTGAAATATGGCGAGG62590.28057589109405773No Hit
GGTATCAACGCAGAGTACTTTTTTT40540.18173105328252276No Hit
GTCCTACAGTGTGCATTTCTCATTT39180.175634500927707No Hit
TATCAACGCAGAGTACTTTTTTTTT38180.17115174184328363No Hit
CTGAAGGACCTGGAATATGGCGAGA33970.15227932609786132No Hit
GTCCTTCAGTGTGCATTTCTCATTT29580.1326000137172428No Hit
CTGTAGGACCTGGAATATGGCGAGA29340.1315241515369812No Hit
ATTTAGAAATGTCCACTGTAGGACG28210.1264586337715828No Hit
GAATATGGCAAGAAAACTGAAAATC25670.11507242569714748No Hit
TTTCTAAATTTTCCACCTTTTTCAG23780.10660001102758734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG400.00534158514.225139
GCCGTGC551.9922935E-413.7922048
GGTATCA12800.013.0652741
TAGGACC12950.012.1812914
CCCGTGT752.1111505E-411.378578
TCCAACG2350.011.32002218
GGCGAGG17950.011.22547419
TGTAGGA42200.011.2145462
GGACGTA855.3098433E-511.17966
TATTGCG600.00586378411.0866865
GTGTAGG4900.011.0533671
GTATTAG1502.1263986E-910.7689531
CACCTTT9900.010.66002514
GTAGGAC43350.010.6539813
GTCCTAA21500.010.6069031
CTGTAGG40050.010.5815221
GTGGTAT4150.010.5323561
CCACCTT10050.010.49452913
AGGACGT42450.010.4766665
GTTTAGG3000.010.4522191
Produced by FastQC (version 0.11.5)