FastQCFastQC Report
Thu 26 May 2016
SRR1512316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230769
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81330.3645827963361513No Hit
CTGTAGGACGTGGAATATGGCAAGA81110.3635965893375782No Hit
GTATCAACGCAGAGTACTTTTTTTT78380.35135865703710245No Hit
GTCCTAAAGTGTGTATTTCTCATTT74560.33423451733460524No Hit
CTTTAGGACGTGAAATATGGCGAGG66760.29926899647610306No Hit
TATCAACGCAGAGTACTTTTTTTTT49070.219968988272654No Hit
GGTATCAACGCAGAGTACTTTTTTT48850.21898278127408083No Hit
GTCCTACAGTGTGCATTTCTCATTT40460.1813724325557689No Hit
CTGAAGGACCTGGAATATGGCGAGA38030.17047932798062015No Hit
CTGTAGGACCTGGAATATGGCGAGA32600.14613794615220133No Hit
ATTTAGAAATGTCCACTGTAGGACG29560.13251035853555435No Hit
GTCCTTCAGTGTGCATTTCTCATTT28920.1296413927215234No Hit
TTTCTAAATTTTCCACCTTTTTCAG28070.12583104749976354No Hit
GAATATGGCAAGAAAACTGAAAATC26940.12076552973436513No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25340.11359311519928779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.014.1066561
TCCAACG2750.013.11861518
TAGGACC12050.013.089954
TCACGAC604.0858513E-412.669393
CCGAATG604.1161082E-412.65831318
CCAACGA1600.012.46080619
CCAACGT1353.765308E-1011.95534119
CACCTTT8800.011.11397814
TGCGGTA600.005876446611.08347712
ACCTTTT8500.011.05889615
AGGACCT24200.010.9553255
GTACTAA1408.18909E-910.8711841
TGTCGAG1502.1573214E-910.75956518
GGCGAGG16900.010.72984719
TAGAAAT10550.010.7179424
GTGCGTA803.761457E-410.6907569
GTCGAGA803.7943403E-410.68069119
AATGTCC10150.010.5800528
GGACCTG24400.010.5549176
ACGAAAT2800.010.51799412
Produced by FastQC (version 0.11.5)