FastQCFastQC Report
Thu 26 May 2016
SRR1512315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823658
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65850.36108744073724347No Hit
GGTATCAACGCAGAGTACTTTTTTT40090.2198328853326665No Hit
TATCAACGCAGAGTACTTTTTTTTT36420.19970849797494925No Hit
GTCCTACAGTGGACATTTCTAAATT34590.1896737217175589No Hit
GTCCTAAAGTGTGTATTTCTCATTT34360.188412520330018No Hit
CTGTAGGACGTGGAATATGGCAAGA31070.17037185700389No Hit
CTTTAGGACGTGAAATATGGCGAGG27800.15244086336363508No Hit
GTCCTACAGTGTGCATTTCTCATTT24300.1332486683358393No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18450.10117028521795204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT1055.456968E-1215.38291556
CGTGCGC653.4139757E-614.59833510
GGTATCA12350.014.308731
TGCGGAC400.005320593714.23337710
GCGCCAC1051.9945219E-812.66653713
GCGTGCG751.5069583E-512.6435379
AGGCGTG1251.4078978E-912.1618877
GGCGTGC951.0654167E-611.97610958
TAGGACC7800.011.6944464
GTTTAGG1751.8189894E-1211.4008271
GGCGAGG7850.011.26490919
TGTAGGA21750.011.2273412
AGAACCG700.001489337910.8591275
GGTATAC803.7688029E-410.6882751
GTAGGAC21850.010.6106353
GCACCGT1002.3956014E-510.4513356
CTGTAGG21000.010.4507591
GTGCGCC1002.425653E-510.43953511
GTCCTAC18400.010.4301031
AGGACCT13400.010.2108215
Produced by FastQC (version 0.11.5)